Takahashi N, Ishihara K, Kimizuka R, Okuda K, Kato T
Oral Health Science Centre, Tokyo Dental College, Masago, Chiba, Japan.
Oral Microbiol Immunol. 2006 Dec;21(6):366-71. doi: 10.1111/j.1399-302X.2006.00305.x.
Prevotella intermedia, a black-pigmented, anaerobic, gram-negative bacterium, is associated with various type of periodontitis. Antibiotic treatments via a systemic or local route have been reported as being useful for treating periodontal disease. The purpose of this study was to examine the effects of four antibiotics, tetracycline (TET), minocycline (MINO), doxycycline (DOXY) and ofloxacin (OFLX) on P. intermedia biofilms at minimum inhibitory concentrations (MIC) from one-fold to 100-fold. MICs were determined for planktonic cells. Biofilm formation was determined with the crystal violet stain method and the bioactivities in the biofilms were determined with the adenosine triphosphate (ATP) -bioluminescent assay using a 96-well culture plate. At one-fold MIC, DOXY inhibited biofilm formation by P. intermedia ATCC 25611. Other antibiotics at one-fold MIC had no effects on the biofilm formation of tested bacterial strains. In P. intermedia ATCC 25611 biofilms, all the antibiotics tested showed inhibitory activities at five- to 100-fold MICs. In the biofilms of P. intermedia strains, except ATCC 25611, treated with three tetracycline antibiotics, the bioactivities were significantly increased, indicating the difficulties involved in designing antibiotic therapy for periodontal disease.
中间普氏菌是一种产黑色素的厌氧革兰氏阴性菌，与多种类型的牙周炎有关。据报道，通过全身或局部途径进行抗生素治疗对治疗牙周疾病有效。本研究的目的是检测四种抗生素，即四环素（TET）、米诺环素（MINO）、多西环素（DOXY）和氧氟沙星（OFLX）在最低抑菌浓度（MIC）从1倍到100倍时对中间普氏菌生物膜的影响。测定浮游细胞的MIC。采用结晶紫染色法测定生物膜形成，并使用96孔培养板通过三磷酸腺苷（ATP）-生物发光测定法测定生物膜中的生物活性。在1倍MIC时，DOXY抑制中间普氏菌ATCC 25611的生物膜形成。其他抗生素在1倍MIC时对受试菌株的生物膜形成没有影响。在中间普氏菌ATCC 25611生物膜中，所有受试抗生素在5倍到100倍MIC时均显示出抑制活性。在用三种四环素类抗生素处理的中间普氏菌菌株（ATCC 25611除外）的生物膜中，生物活性显著增加，这表明设计牙周疾病抗生素治疗存在困难。
