Jeraci J L, Lewis B A, Robertson J B, Van Soest P J
Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853.
Adv Exp Med Biol. 1990;270:311-20. doi: 10.1007/978-1-4684-5784-1_28.
Total dietary fiber (TDF) values for cereal grains, fruits, vegetables, processed foods, and purified or semi-purified dietary fiber products were determined by a new method using 8M urea and enzymes (urea enzymatic dialysis, UED, method). The results are compared with the official AOAC procedure. Soluble and insoluble dietary fiber were determined for several of these foodstuffs and compared with the NDF values. Crude protein and ash contamination was usually lower with the UED method compared with the AOAC method, particularly for samples that formed gels during ethanol precipitation. Urea and the heat stable amylase were effective in removing starch even at relatively low temperatures of the assay (50 degrees C). The new assay is relatively economical in use of equipment, enzymes, and reagents. Studies are currently in progress to minimize the assay time for the UED method while further improving its flexibility and robustness. The results of the studies will be discussed.
采用一种使用8M尿素和酶的新方法（尿素酶促透析法，即UED法）测定了谷物、水果、蔬菜、加工食品以及纯化或半纯化膳食纤维产品中的总膳食纤维（TDF）值。将结果与官方AOAC方法进行了比较。测定了其中几种食品的可溶性和不溶性膳食纤维，并与中性洗涤纤维（NDF）值进行了比较。与AOAC方法相比，UED方法的粗蛋白和灰分污染通常较低，特别是对于在乙醇沉淀过程中形成凝胶的样品。即使在相对较低的测定温度（50摄氏度）下，尿素和热稳定淀粉酶也能有效去除淀粉。新方法在设备、酶和试剂的使用上相对经济。目前正在进行研究，以尽量缩短UED方法的测定时间，同时进一步提高其灵活性和稳健性。将讨论研究结果。
