Suzuki Rikako, Kohno Hiroyuki, Yasui Yumiko, Hata Kazuya, Sugie Shigeyuki, Miyamoto Shingo, Sugawara Kuniaki, Sumida Takashi, Hirose Yoshinobu, Tanaka Takuji
Department of Oncologic Pathology, Kanazawa Medical University, Ishikawa, Japan.
Int J Cancer. 2007 Jan 15;120(2):252-8. doi: 10.1002/ijc.22240.
The modulatory effects of dietary citrus unshiu segment membrane (CUSM) on the occurrence of aberrant crypt foci (ACF) and beta-catenin accumulated crypts (BCACs) were determined in male C57BL/KsJ-db/db (db/db) mice initiated with azoxymethane (AOM). Male db/db, db/+ and +/+ mice were given 5 weekly subcutaneous injections of AOM (15 mg/kg body weight), and then they were fed the diet containing 0.02%, 0.1% or 0.5% CUSM for 7 weeks. At Week 12, a significant increase in the numbers of ACF and BCAC was noted in the db/db mice in comparison with the db/+ and +/+ mice. Feeding with CUSM caused reduction in the frequency of ACF in all genotypes of mice and the potency was high in order of the db/db mice, db/+ mice and +/+ mice. The number of BCACs was also reduced by feeding with CUSM, thus resulting in a 28-61% reduction in the db/db mice, possibly due to suppression of cell proliferation activity in the lesions by feeding with CUSM-containing diet. Clinical chemistry revealed a low serum level of triglyceride in mice fed CUSM. In addition, CUSM feeding inhibited fatty metamorphosis and fibrosis in the liver of db/db mice. Our findings show that CUSM in the diet has a chemopreventive ability against the early phase of AOM-induced colon carcinogenesis in the db/db as well as db/+ and +/+ mice, indicating potential use of CUSM in cancer chemoprevention in obese people.
在以氧化偶氮甲烷（AOM）启动的雄性C57BL/KsJ-db/db（db/db）小鼠中，确定了膳食温州蜜柑囊衣（CUSM）对异常隐窝灶（ACF）和β-连环蛋白积聚隐窝（BCACs）发生的调节作用。雄性db/db、db/+和+/+小鼠每周皮下注射5次AOM（15毫克/千克体重），然后给它们喂食含0.02%、0.1%或0.5%CUSM的饲料7周。在第12周时，与db/+和+/+小鼠相比，db/db小鼠的ACF和BCAC数量显著增加。用CUSM喂养导致所有基因型小鼠的ACF频率降低，效力顺序为db/db小鼠、db/+小鼠和+/+小鼠。用CUSM喂养也减少了BCAC的数量，从而使db/db小鼠减少了28 - 61%，这可能是由于含CUSM的饮食抑制了病变中的细胞增殖活性。临床化学检测显示，喂食CUSM的小鼠血清甘油三酯水平较低。此外，喂食CUSM可抑制db/db小鼠肝脏中的脂肪变性和纤维化。我们的研究结果表明，饮食中的CUSM对db/db以及db/+和+/+小鼠中AOM诱导的结肠癌发生早期具有化学预防能力，表明CUSM在肥胖人群癌症化学预防中具有潜在用途。
