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荆芥植物精油及其成分对茹氏尖眼蕈蚊(双翅目:眼蕈蚊科)的熏蒸活性

Fumigant activity of plant essential oils and components from Schizonepeta tenuifolia against Lycoriella ingenua (Diptera: Sciaridae).

作者信息

Park Il-Kwon, Kim Lee-Sum, Choi In-Ho, Lee Yeon-Suk, Shin Sang-Chul

机构信息

Division of Forest Insect Pests and Diseases, Korea Forest Research Institute, Seoul 130-012, Republic of Korea.

出版信息

J Econ Entomol. 2006 Oct;99(5):1717-21. doi: 10.1603/0022-0493-99.5.1717.

DOI:10.1603/0022-0493-99.5.1717
PMID:17066804
Abstract

Plant essential oils from 21 plant species were tested for their insecticidal activities against larvae of Lycoriella ingenua Dufour (Diptera: Sciaridae) by using a fumigation bioassay. Good insecticidal activity against larvae of L. ingenua was achieved with essential oils of Acorus gramineus Solander, Schizonepeta tenuifolia Briquet, and Zanthoxylum piperitum De Candolle at 25 microg/ml air. S. tenuifolia oil showed the most potent insecticidal activity among the plant essential oils. At 12.5 microg/ml air concentration, S. tenuifolia oil caused 96.6% mortality, but mortality decreased to 60% at 3.125 microg/ml air. Analysis by gas chromatography-mass spectrometry led to identification of three major compounds from S. tenuifolia oil. These three compounds were tested individually for their insecticidal activities against larvae of L. ingenua and compared with the toxicity of dichlorvos. Pulegone was the most toxic, followed by menthone and limonene with LC50 values of 1.21, 6.03, and 15.42 microg/ml, respectively. LC50 of dichlorvos was 8.13 microg/ml. Effects of S. tenuifolia and its components on growth of Pleurotus ostreatus (Jacq. ex Fr.) Kummer also were investigated.

摘要

采用熏蒸生物测定法,对21种植物的植物精油针对嗜菇瘿蚊(双翅目:眼蕈蚊科)幼虫的杀虫活性进行了测试。石菖蒲、荆芥和花椒的精油在空气浓度为25微克/毫升时,对嗜菇瘿蚊幼虫具有良好的杀虫活性。荆芥精油在植物精油中表现出最强的杀虫活性。在空气浓度为12.5微克/毫升时,荆芥精油导致96.6%的死亡率,但在空气浓度为3.125微克/毫升时,死亡率降至60%。通过气相色谱-质谱分析鉴定出荆芥精油中的三种主要化合物。分别测试了这三种化合物对嗜菇瘿蚊幼虫的杀虫活性,并与敌敌畏的毒性进行了比较。长叶薄荷酮毒性最大,其次是薄荷酮和柠檬烯,其半数致死浓度(LC50)值分别为1.21、6.03和15.42微克/毫升。敌敌畏的LC50为8.13微克/毫升。还研究了荆芥及其成分对糙皮侧耳生长的影响。

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