尿液中N,N-二甲基甘氨酸的气相色谱测定法。

Gas chromatographic assay for N,N-dimethylglycine in urine.

作者信息

Tjørnelund J, Hansen S H

机构信息

Department of Organic Chemistry, Royal Danish School of Pharmacy, Copenhagen.

出版信息

J Chromatogr. 1990 Sep 14;530(2):227-34. doi: 10.1016/s0378-4347(00)82327-0.

DOI:10.1016/s0378-4347(00)82327-0

PMID:1706726

Abstract

A gas chromatographic method for the determination of N,N-dimethylglycine in urine has been developed. After clean-up by cation-exchange, N,N-dimethylglycine was derivatized with ethanol and hydrochloric acid to form the corresponding ethyl ester. After evaporation of solvent, N,N-dimethylglycine ethyl ester was extracted into methylene chloride and chromatographed on a gas chromatograph equipped with a packed column containing 10% Carbowax 20 M. The detection limit of the method is 0.01 mM N,N-dimethylglycine in urine. This method has been used to detect N,N-dimethylglycine in urine from healthy subjects as well as in urine from patients with metabolic disorders. These findings were verified by gas chromatography-mass spectrometry.

摘要

已开发出一种气相色谱法用于测定尿液中的N,N-二甲基甘氨酸。通过阳离子交换进行净化后，N,N-二甲基甘氨酸用乙醇和盐酸衍生化以形成相应的乙酯。溶剂蒸发后，将N,N-二甲基甘氨酸乙酯萃取到二氯甲烷中，并在配备有填充10%聚乙二醇20M的填充柱的气相色谱仪上进行色谱分析。该方法的检测限为尿液中0.01 mM的N,N-二甲基甘氨酸。此方法已用于检测健康受试者尿液以及患有代谢紊乱患者尿液中的N,N-二甲基甘氨酸。这些结果通过气相色谱-质谱联用得到了验证。