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使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和rRNA限制性图谱鉴定凝固酶阴性葡萄球菌。

Identification of coagulase-negative staphylococci by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and rRNA restriction patterns.

作者信息

Pennington T H, Harker C, Thomson-Carter F

机构信息

Department of Medical Microbiology, University of Aberdeen, Scotland.

出版信息

J Clin Microbiol. 1991 Feb;29(2):390-2. doi: 10.1128/jcm.29.2.390-392.1991.

Abstract

A total of 1,417 staphylococcal and micrococcal strains were collected from the beards and scalps of 10 subjects over a period of 8 months. Sixteen strains identified as Staphylococcus epidermidis with an API system had distinctive yellow colonies on nutrient agar plates and sodium dodecyl sulfate-polyacrylamide gel electrophoresis whole-cell polypeptide profiles similar to those of Staphylococcus capitis; this identification was confirmed by analysis of rRNA gene restriction patterns.

摘要

在8个月的时间里,从10名受试者的胡须和头皮上共收集了1417株葡萄球菌和微球菌菌株。用API系统鉴定为表皮葡萄球菌的16株菌株在营养琼脂平板上有独特的黄色菌落,其十二烷基硫酸钠-聚丙烯酰胺凝胶电泳全细胞多肽图谱与头葡萄球菌相似;通过rRNA基因限制性图谱分析证实了这一鉴定结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9d4/269773/45cc5457c21c/jcm00038-0175-a.jpg

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