通过核糖体RNA基因限制性图谱对葡萄球菌菌种和菌株进行鉴别

Differentiation of staphylococcal species and strains by ribosomal RNA gene restriction patterns.

作者信息

Thomson-Carter F M, Carter P E, Pennington T H

机构信息

Department of Medical Microbiology, University of Aberdeen Medical School, Foresterhill, UK.

出版信息

J Gen Microbiol. 1989 Jul;135(7):2093-7. doi: 10.1099/00221287-135-7-2093.

DOI:10.1099/00221287-135-7-2093

PMID:2482338

Abstract

Staphylococcal DNA was digested with endonucleases and probed with labelled ribosomal RNA (rRNA) from Escherichia coli. Reproducible restriction patterns containing between seven and 22 bands were obtained for seven different species of staphylococci. These profiles were species-specific with different strains of a particular species sharing an identical or similar restriction pattern. The results reported here indicate that rRNA gene restriction pattern analyses have an application in the taxonomy of staphylococci.

摘要

用核酸内切酶消化葡萄球菌DNA，并用来自大肠杆菌的标记核糖体RNA（rRNA）进行探测。对七种不同的葡萄球菌物种获得了包含7至22条带的可重复限制性图谱。这些图谱具有物种特异性，特定物种的不同菌株具有相同或相似的限制性图谱。此处报道的结果表明，rRNA基因限制性图谱分析在葡萄球菌分类学中有应用。