Kloos W E, Schleifer K H
J Clin Microbiol. 1975 Jan;1(1):82-8. doi: 10.1128/jcm.1.1.82-88.1975.
From a total of 40 characters that were previously used to differentiate species of staphylococci, 13 key characters were selected to make a simplified scheme that could be easily used by the routine clinical laboratory for identifying human staphylococci. These key characters included coagulase activity, hemolysis, nitrate reduction, and aerobic acid production from fructose, xylose, arabinose, ribose, maltose, lactose, sucrose, trehalose, mannitol, and xylitol. In the simplified scheme, 924 strains of staphylococci were placed into 11 positions, each of which contained the major portion (greater than or equal to 80%) of strains of one of the recognized species. Several positions contained a rare or few uncommon strains of one or more additional species and these could be resolved on the basis of other key characters.
从先前用于区分葡萄球菌种类的总共40个特征中,选取了13个关键特征,制定了一个简化方案,常规临床实验室可轻松使用该方案来鉴定人类葡萄球菌。这些关键特征包括凝固酶活性、溶血、硝酸盐还原以及从果糖、木糖、阿拉伯糖、核糖、麦芽糖、乳糖、蔗糖、海藻糖、甘露醇和木糖醇中产酸需氧。在简化方案中,924株葡萄球菌被归入11个类别,每个类别包含一种已确认葡萄球菌种类的大部分菌株(大于或等于80%)。有几个类别包含一种或多种其他葡萄球菌种类的罕见或少量不常见菌株,这些菌株可根据其他关键特征来区分。