Adinolfi Barbara, Chicca Andrea, Martinotti Enrica, Breschi Maria C, Nieri Paola
Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, University of Pisa, Italy.
Fitoterapia. 2007 Jan;78(1):43-5. doi: 10.1016/j.fitote.2006.09.012. Epub 2006 Sep 23.
In our previous study, RAPD (Random Amplified Polymorphic DNA) analysis revealed species-specific markers for three medicinal Echinacea species (Asteraceae): E. angustifolia DC., E. pallida (Nutt.) Nutt. and E. purpurea (L.) Moench. In the present work, we have converted a RAPD marker (750 bp) for E. purpurea into a SCAR (Sequence Characterized Amplified Region) marker. SCAR-PCR, in fact, revealed the expected amplicon (330 bp) only in E. purpurea and not in the other two species, giving further evidence for differences in medicinal Echinacea spp. genome and confirming a greater similarity between E. pallida and angustifolia.
在我们之前的研究中，随机扩增多态性DNA（RAPD）分析揭示了三种药用紫锥菊属植物（菊科）的物种特异性标记：狭叶紫锥菊（E. angustifolia DC.）、淡紫锥菊（E. pallida (Nutt.) Nutt.）和紫花紫锥菊（E. purpurea (L.) Moench.）。在本研究中，我们已将紫花紫锥菊的一个RAPD标记（750 bp）转化为序列特异性扩增区域（SCAR）标记。事实上，SCAR-PCR仅在紫花紫锥菊中揭示了预期的扩增子（330 bp），而在其他两个物种中未出现，这进一步证明了药用紫锥菊属植物基因组的差异，并证实了淡紫锥菊和狭叶紫锥菊之间具有更高的相似性。
