Hirose S, Donoso L A, Shinohara T, Palestine A G, Nussenblatt R B, Gery I
Department of Ophthalmology, Hokkaido University School of Medicine, Japan.
Jpn J Ophthalmol. 1990;34(3):298-305.
A synthetic amino acid, peptide M18 (18 amino acids in length which correspond to the amino acid positions 303-322 of bovine S-antigen: DTNLASSTIIKEGIDKTV), is capable of inducing experimental autoimmune uveitis in monkeys and Hartley guinea pigs as well as Lewis rats. Recently, the amino acid sequence of human S-antigen corresponding to peptide M was found to be virtually identical to that of bovine S-antigen. To investigate the role of the epitope corresponding to peptide M in human uveitis, 21 patients with uveitis were tested for proliferation of peripheral mononuclear cells against peptide M18 and bovine S-antigen. Six of the 21 patients responded to S-antigen and 4 of these 6 patients responded to peptide M18. These findings show that peptide M18 is immunogenic in man and provide additional support to the hypothesis that the amino acid sequence which corresponds to peptide M might be involved in uveitic conditions in man.
一种合成氨基酸肽M18（长度为18个氨基酸，对应牛S抗原的303 - 322位氨基酸：DTNLASSTIIKEGIDKTV），能够在猴子、哈特利豚鼠以及刘易斯大鼠中诱发实验性自身免疫性葡萄膜炎。最近发现，与肽M对应的人S抗原的氨基酸序列与牛S抗原的氨基酸序列几乎相同。为了研究与肽M对应的表位在人类葡萄膜炎中的作用，对21例葡萄膜炎患者进行了外周血单个核细胞针对肽M18和牛S抗原的增殖检测。21例患者中有6例对S抗原有反应，这6例患者中有4例对肽M18有反应。这些发现表明肽M18在人体中具有免疫原性，并为与肽M对应的氨基酸序列可能参与人类葡萄膜炎病情的假说提供了更多支持。
