Nityanand S, Singh V K, Shinohara T, Paul A K, Singh V, Agarwal P K, Agarwal S S
Department of Immunology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow, India.
J Clin Immunol. 1993 Sep;13(5):352-8. doi: 10.1007/BF00920244.
Peptide M, an 18-amino acid fragment from position 303 to position 320 of retinal S-antigen, produces experimental autoimmune uveitis (EAU), similar to that produced by native S-antigen, in several vertebrate species including nonhuman primates. It was observed that 12 of the 39 (30.7%) patients with uveitis, 1 of the 29 (3.4%) patients with systemic connective tissue disorders (CTD) without eye involvement, 2 of the 7 (5.8%) patients of CTD with uveitis, 1 of the 17 (5.8%) patients with diabetic retinopathy, and none of the 19 normal healthy controls showed a significant lymphoproliferative response to peptide M (stimulation index of 3 or more). Yeast histone H3 peptide gave a positive response in 1 (2.5%), 2 (6.8%), 1 (14.2%), 2 (11.7%), and 2 (10.5%) individuals, respectively, in the different groups studied. In a few cases a positive response to yeast histone H3 peptide was observed without significant stimulation to peptide M. These findings indicate that peptide M could also be an immunogenic epitope of S-antigen in humans and be aetiopathologically related to uveitis in a subset of patients with this disease. However, unlike experimental animals, the responses to peptide M and yeast histone H3 were nonconcordant, necessitating further studies.
肽M是视网膜S抗原303位至320位的一个18氨基酸片段，在包括非人灵长类动物在内的几种脊椎动物中，可引发实验性自身免疫性葡萄膜炎（EAU），类似于天然S抗原引发的葡萄膜炎。研究发现，39例葡萄膜炎患者中有12例（30.7%）、29例无眼部受累的系统性结缔组织病（CTD）患者中有1例（3.4%）、7例合并葡萄膜炎的CTD患者中有2例（5.8%）、17例糖尿病视网膜病变患者中有1例（5.8%），而19例正常健康对照者中无人对肽M表现出显著的淋巴细胞增殖反应（刺激指数为3或更高）。在不同研究组中，酵母组蛋白H3肽分别在1例（2.5%）、2例（6.8%）、1例（14.2%）、2例（11.7%）和2例（10.5%）个体中引发阳性反应。在少数情况下，观察到对酵母组蛋白H3肽有阳性反应，但对肽M无显著刺激。这些发现表明，肽M在人类中也可能是S抗原的一个免疫原性表位，并且在一部分患有该病的患者中与葡萄膜炎在病因学上相关。然而，与实验动物不同的是，对肽M和酵母组蛋白H3的反应不一致，需要进一步研究。
