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散粒噪声对数字全息显微镜中重建相位图像信噪比的影响。

Shot-noise influence on the reconstructed phase image signal-to-noise ratio in digital holographic microscopy.

作者信息

Charrière Florian, Colomb Tristan, Montfort Frédéric, Cuche Etienne, Marquet Pierre, Depeursinge Christian

机构信息

Imaging and Applied Optics Institute, Ecole Polytechnique Féderale de Lausanne, Switzerland.

出版信息

Appl Opt. 2006 Oct 10;45(29):7667-73. doi: 10.1364/ao.45.007667.

DOI:10.1364/ao.45.007667
PMID:17068602
Abstract

In digital holographic microscopy, shot noise is an intrinsic part of the recording process with the digital camera. We present a study based on simulations and real measurements describing the shot-noise influence in the quality of the reconstructed phase images. Different configurations of the reference wave and the object wave intensities will be discussed, illustrating the detection limit and the coherent amplification of the object wave. The signal-to-noise ratio (SNR) calculation of the reconstructed phase images based on the decision statistical theory is derived from a model for image quality estimation proposed by Wagner and Brown [Phys. Med. Biol. 30, 489 (1985)]. It will be shown that a phase image with a SNR above 10 can be obtained with a mean intensity lower than 10 photons per pixel and per hologram coming from the observed object. Experimental measurements on a glass-chrome probe will be presented to illustrate the main results of the simulations.

摘要

在数字全息显微镜中,散粒噪声是数码相机记录过程中固有的一部分。我们基于模拟和实际测量进行了一项研究,描述了散粒噪声对重建相位图像质量的影响。将讨论参考波和物波强度的不同配置,说明检测极限和物波的相干放大。基于决策统计理论的重建相位图像的信噪比(SNR)计算源自瓦格纳和布朗提出的图像质量估计模型[《物理医学与生物学》30, 489 (1985)]。结果表明,对于来自被观测物体的每个像素和每个全息图,当平均强度低于10个光子时,即可获得信噪比高于10的相位图像。将展示对玻璃铬探针的实验测量结果,以说明模拟的主要结果。

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