Han Chao, Chen Junhui, Chen Bo, Lee Frank Sen-Chun, Wang Xiaoru
Department of Chemistry, Key Laboratory of Analytical Science of the MOE, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, PR China.
J Sep Sci. 2006 Sep;29(14):2197-202. doi: 10.1002/jssc.200600134.
A simple and reliable high performance liquid chromatographic (HPLC) method has been developed and validated for the fingerprinting of extracts from the root of Pseudostellaria heterophylla (Miq.) Pax. HPLC with gradient elution was performed on an authentic reference standard of powdered P. heterophylla (Miq.) Pax root and 11 plant samples of the root were collected from different geographic locations. The HPLC chromatograms have been standardized through the selection and identification of reference peaks and the normalization of retention times and peak intensities of all the common peaks. The standardized HPLC fingerprints show high stability and reproducibility, and thus can be used effectively for the screening analysis or quality assessment of the root or its derived products. Similarity index calculations based on cosine angle values or correlation methods have been performed on the HPLC fingerprints. As a group, the fingerprints of the P. heterophylla (Miq.) Pax samples studied are highly correlated with closely similar fingerprints. Within the group, the samples can be further divided into subgroups based on hierarchical clustering analysis (HCA). Sample grouping based on HCA coincides nicely with those based on the geographical origins of the samples. The HPLC fingerprinting techniques thus have high potential in authentication or source-tracing types of applications.
已开发并验证了一种简单可靠的高效液相色谱（HPLC）方法，用于太子参（Pseudostellaria heterophylla (Miq.) Pax）根提取物的指纹图谱分析。采用梯度洗脱的HPLC法对太子参（Pseudostellaria heterophylla (Miq.) Pax）根粉的正品对照标准品进行分析，并从不同地理位置收集了11份该植物根的样品。通过选择和鉴定参照峰以及对所有共有峰的保留时间和峰强度进行归一化处理，对HPLC色谱图进行了标准化。标准化的HPLC指纹图谱显示出高稳定性和重现性，因此可有效地用于该植物根或其衍生产品的筛选分析或质量评估。基于余弦角值或相关性方法对HPLC指纹图谱进行了相似度指数计算。作为一个整体，所研究的太子参（Pseudostellaria heterophylla (Miq.) Pax）样品的指纹图谱与相似度极高的指纹图谱高度相关。在该组内，基于层次聚类分析（HCA）可将样品进一步分为亚组。基于HCA的样品分组与基于样品地理来源的分组非常吻合。因此，HPLC指纹图谱技术在鉴定或溯源类型的应用中具有很高的潜力。
