Huang Huarong, Zhao Xiaoli, Chen Liangbiao, Xu Chao, Yao Xing, Lu Yongliang, Dai Licheng, Zhang Ming
College of Life Sciences, Zhejiang University, No. 338, Yu-Hang-Tang Road, Hangzhou, Zhejiang 310058, China.
Biochem Biophys Res Commun. 2006 Dec 15;351(2):321-7. doi: 10.1016/j.bbrc.2006.09.171. Epub 2006 Oct 17.
Smooth muscle cell (SMC) plays critical roles in many human diseases, an in vitro system that recapitulates human SMC differentiation would be invaluable for exploring molecular mechanisms leading to the human diseases. We report a directed and highly efficient SMC differentiation system by treating the monolayer-cultivated human embryonic stem cells (hESCs) with all-trans retinoid acid (atRA). When the hESCs were cultivated in differentiation medium containing 10microM RA, more than 93% of the cells expressed SMC-marker genes along with the steadily accumulation of such SMC-specific proteins as SM alpha-actin and SM-MHC. The fully differentiated SMCs were stable in phenotype and capable of contraction. This inducible and highly efficient in vitro human SMC system could be an important resource to study the mechanisms of SMC phenotype determination in human.
平滑肌细胞（SMC）在许多人类疾病中起关键作用，一个能够概括人类SMC分化的体外系统对于探索导致人类疾病的分子机制将是非常宝贵的。我们报告了一种通过用全反式视黄酸（atRA）处理单层培养的人类胚胎干细胞（hESCs）来进行定向且高效的SMC分化系统。当hESCs在含有10微摩尔RA的分化培养基中培养时，超过93%的细胞表达SMC标记基因，同时诸如平滑肌α-肌动蛋白和平滑肌肌球蛋白重链等SMC特异性蛋白稳步积累。完全分化的SMC在表型上是稳定的，并且能够收缩。这种可诱导且高效的体外人类SMC系统可能是研究人类SMC表型确定机制的重要资源。
