Kovacević Nada, Colić Miodrag, Backović Aleksandar, Doslov-Kokorus Zvezdana
Department of Pharmacognosy, Faculty of Pharmacy, Vojvode Stepe 450, 11000 Belgrade, Serbia and Montenegro.
Fitoterapia. 2006 Dec;77(7-8):561-7. doi: 10.1016/j.fitote.2006.09.008. Epub 2006 Sep 22.
The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 microg/ml and 1 microg/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 microg/ml-500 microg/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2Ralpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2Ralpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ralpha expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 microg/ml could not be restored by the addition of exogenous IL-2.
研究了高山淫羊藿根及根茎甲醇提取物（MEEA）对大鼠淋巴细胞表型和功能的体外影响。结果发现，较低浓度（0.1微克/毫升和1微克/毫升）的MEEA能显著增强伴刀豆球蛋白A（Con A）触发的脾细胞和胸腺细胞增殖，而较高浓度的提取物（50微克/毫升 - 500微克/毫升）则具有抑制作用。MEEA对Con A诱导的脾细胞增殖的刺激作用与白细胞介素-2受体α（IL-2Rα）表达上调相关。此外，当向细胞培养物中加入抗IL-2Rα单克隆抗体（mAb）时，观察到IL-2产生增加。MEEA抑制脾细胞增殖是由于抑制IL-2产生、下调IL-2Rα表达以及诱导细胞凋亡。添加外源性IL-2无法恢复高于50微克/毫升的抑制浓度MEEA存在时的细胞增殖。
