裂谷热病毒的NSm蛋白对于病毒成熟、复制和感染并非必需。
The NSm proteins of Rift Valley fever virus are dispensable for maturation, replication and infection.
作者信息
Gerrard Sonja R, Bird Brian H, Albariño Cesar G, Nichol Stuart T
机构信息
Department of Epidemiology, School of Public Health, University of Michigan, 109 S. Observatory, Ann Arbor, MI 48109, USA.
出版信息
Virology. 2007 Mar 15;359(2):459-65. doi: 10.1016/j.virol.2006.09.035. Epub 2006 Oct 30.
Abstract
Rift Valley fever (RVF) virus belongs to the Bunyaviridae family of segmented negative-strand RNA viruses and causes mosquito-borne disease in sub-Saharan Africa. We report the development of a T7 RNA polymerase-driven plasmid-based genetic system for the virulent Egyptian isolate, ZH501. We have used this system to rescue a virus that has a 387 nucleotide deletion on the genomic M segment that eliminates the coding region for two non-structural proteins known as NSm. This virus, DeltaNSm rZH501, is indistinguishable from the parental ZH501 strain with respect to expression of structural proteins and growth in cultured mammalian cells.
摘要
裂谷热（RVF）病毒属于布尼亚病毒科的分节段负链RNA病毒，在撒哈拉以南非洲地区引发蚊媒疾病。我们报告了一种基于T7 RNA聚合酶驱动的质粒遗传系统的开发，该系统用于强毒株埃及分离株ZH501。我们利用这个系统拯救了一种病毒，该病毒在基因组M片段上有387个核苷酸的缺失，消除了两种被称为NSm的非结构蛋白的编码区域。这种病毒，DeltaNSm rZH501，在结构蛋白表达和在培养的哺乳动物细胞中的生长方面与亲本ZH501株没有区别。
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