Gerrard Sonja R, Bird Brian H, Albariño Cesar G, Nichol Stuart T
Department of Epidemiology, School of Public Health, University of Michigan, 109 S. Observatory, Ann Arbor, MI 48109, USA.
Virology. 2007 Mar 15;359(2):459-65. doi: 10.1016/j.virol.2006.09.035. Epub 2006 Oct 30.
Rift Valley fever (RVF) virus belongs to the Bunyaviridae family of segmented negative-strand RNA viruses and causes mosquito-borne disease in sub-Saharan Africa. We report the development of a T7 RNA polymerase-driven plasmid-based genetic system for the virulent Egyptian isolate, ZH501. We have used this system to rescue a virus that has a 387 nucleotide deletion on the genomic M segment that eliminates the coding region for two non-structural proteins known as NSm. This virus, DeltaNSm rZH501, is indistinguishable from the parental ZH501 strain with respect to expression of structural proteins and growth in cultured mammalian cells.
裂谷热（RVF）病毒属于布尼亚病毒科的分节段负链RNA病毒，在撒哈拉以南非洲地区引发蚊媒疾病。我们报告了一种基于T7 RNA聚合酶驱动的质粒遗传系统的开发，该系统用于强毒株埃及分离株ZH501。我们利用这个系统拯救了一种病毒，该病毒在基因组M片段上有387个核苷酸的缺失，消除了两种被称为NSm的非结构蛋白的编码区域。这种病毒，DeltaNSm rZH501，在结构蛋白表达和在培养的哺乳动物细胞中的生长方面与亲本ZH501株没有区别。
