Jouanneau J, Gavrilovic J, Caruelle D, Jaye M, Moens G, Caruelle J P, Thiery J P
Laboratoire de Physiopathologie du Développement, Centre National de la Recherche Scientifique Unite de Recherche Associeé 1337, Paris, France.
Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2893-7. doi: 10.1073/pnas.88.7.2893.
Addition of exogenous acidic fibroblast growth factor (aFGF) to NBT-II epithelial carcinoma cells results in fibroblastic transformation and cell motility. We have generated aFGF-producing NBT-II cells by transfection with recombinant expression vectors containing human aFGF cDNA, or the human aFGF cDNA coupled to a signal peptide (SP) sequence. The effects of the nonsecreted and the secreted 16-kDa growth factor on the morphology, motility, and cell invasive potential (gelatinase activity) were compared. aFGF coupled to a SP was actively secreted out of the producing cells. The secretion of aFGF was not necessary for induction of gelatinase activity, as this was observed in NBT-II cells producing aFGF with or without SP. Production of aFGF, whether secreted or not secreted, resulted in increased in vitro motility of most isolated clones; however, there was no correlation between aFGF level and motility rate. The data suggest that expression of aFGF in NBT-II cells induces metastatic potential through an autocrine or intracrine mechanism.
向NBT-II上皮癌细胞中添加外源性酸性成纤维细胞生长因子（aFGF）会导致成纤维细胞转化和细胞运动。我们通过用含有人类aFGF cDNA或与信号肽（SP）序列偶联的人类aFGF cDNA的重组表达载体转染，生成了产生aFGF的NBT-II细胞。比较了非分泌型和分泌型16 kDa生长因子对形态、运动和细胞侵袭潜能（明胶酶活性）的影响。与SP偶联的aFGF被主动分泌到产生细胞外。aFGF的分泌对于诱导明胶酶活性并非必需，因为在产生有或没有SP的aFGF的NBT-II细胞中都观察到了这种活性。无论aFGF是否分泌，其产生都会导致大多数分离克隆的体外运动增加；然而，aFGF水平与运动速率之间没有相关性。数据表明，NBT-II细胞中aFGF的表达通过自分泌或内分泌机制诱导转移潜能。
