Ishii Nobuyoshi, Uchida Shigeo
Office of Biospheric Assessment for Waste Disposal, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba-shi, 263-8555 Japan.
J Environ Qual. 2006 Oct 27;35(6):2017-20. doi: 10.2134/jeq2006.0045. Print 2006 Nov-Dec.
Based on limited data for the removal of radioactive (99)Tc by freshwater phytoplankton, it has been thought that phytoplankton are unsuitable for remediation of (99)Tc-contaminated waters. This work sought to verify the validity of this assumption by studying the removal of (99)TcO(4)(-) by freshwater and brackish water phytoplankton. The phytoplankton used were Euglena gracilis, Chlamydomonas pulsatilla, Chlorella vulgaris, and Spirulina platensis. Each of them was incubated for 63 d, and the removal of (99)Tc from solution was periodically determined. Significant removal of (99)Tc was observed only for E. gracilis, and the maximum removal was 70% of the total (99)Tc added. The killed cells of E. gracilis, however, removed hardly any (99)Tc. When E. gracilis cells were washed with fresh culture medium, only 13% of the total (99)Tc was desorbed. These results suggested that intracellular uptake of (99)Tc by E. gracilis occurred. These results are the first documented example of significant removal of (99)Tc by planktonic microalgae.
基于淡水浮游植物对放射性(99)Tc去除的有限数据,人们一直认为浮游植物不适用于修复受(99)Tc污染的水体。这项工作旨在通过研究淡水和微咸水浮游植物对(99)TcO4(-)的去除来验证这一假设的有效性。所用的浮游植物有纤细裸藻、脉动衣藻、普通小球藻和钝顶螺旋藻。将它们各自培养63天,并定期测定溶液中(99)Tc的去除情况。仅观察到纤细裸藻对(99)Tc有显著去除,最大去除量为添加的总(99)Tc的70%。然而,纤细裸藻的死细胞几乎没有去除任何(99)Tc。当用新鲜培养基洗涤纤细裸藻细胞时,仅13%的总(99)Tc被解吸。这些结果表明纤细裸藻对(99)Tc发生了细胞内摄取。这些结果是浮游微藻显著去除(99)Tc的首个有记录的实例。