Robbe Catherine, Michalski Jean-Claude, Capon Calliope
Unité de Glycobiologie Structurale et Funtionelle, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, France.
Methods Mol Biol. 2006;347:109-23. doi: 10.1385/1-59745-167-3:109.
Nano-electrospray ionization quadrupole time-of-flight mass spectrometry (nanoESI-Q-TOF-MS) provides a sensitive means for mapping and sequencing underivatized O-glycans. This chapter describes fragmentation rules of O-glycans by ESI-MS/MS and provides a series of diagnostic ions relevant for the determination of the core type, position, and linkage of fucose, sialic acid, and sulphate residues, as well as information on type I or II chains. Positive-ion mode gives information about core type, linkage, and position of fucose residues. Negative-ion mode can be applied for differentiation between isomeric molecules and for analysis of sulphated or sialylated glycans. The current technology successfully determines the sequence of underivatized oligosaccharides in complex mixtures and provides a significant step toward the goal of characterizing all aspects of carbohydrate structure using a single instrument.
纳升电喷雾电离四极杆飞行时间质谱（nanoESI-Q-TOF-MS）为未衍生化O-聚糖的图谱绘制和测序提供了一种灵敏的方法。本章描述了通过电喷雾串联质谱（ESI-MS/MS）分析O-聚糖的裂解规则，并提供了一系列用于确定岩藻糖、唾液酸和硫酸根残基的核心类型、位置和连接方式的诊断离子，以及关于I型或II型链的信息。正离子模式可提供有关岩藻糖残基的核心类型、连接方式和位置的信息。负离子模式可用于区分异构体分子以及分析硫酸化或唾液酸化聚糖。当前技术成功地确定了复杂混合物中未衍生化寡糖的序列，并朝着使用单一仪器表征碳水化合物结构各个方面的目标迈出了重要一步。
