Chen Man, Yu Yuanyuan, Jiang Feng, Zhou Junwei, Li Yongshu, Liang Chao, Dang Lei, Lu Aiping, Zhang Ge
Institute of Integrated Bioinformedicine and Translational Science, School of Chinese Medicine, Hong Kong Baptist University (HKBU), Hong Kong 999077, China.
Institute of Precision Medicine and Innovative Drug Discovery, HKBU (Haimen) Institute of Science and Technology, Haimen 226100, China.
Int J Mol Sci. 2016 Dec 10;17(12):2079. doi: 10.3390/ijms17122079.
SELEX (systematic evolution of ligands by exponential enrichment) is a process involving the progressive isolation of high selective ssDNA/RNA from a combinatorial single-stranded oligonucleotide library through repeated rounds of binding, partitioning and amplification. SELEX-derived single-stranded DNA/RNA molecules, called aptamers, are selected against a wide range of targets, including purified proteins, live cells, tissues, microorganisms, small molecules and so on. With the development of SELEX technology over the last two decades, various modified SELEX processes have been arisen. A majority of aptamers are selected against purified proteins through traditional SELEX. Unfortunately, more and more evidence showed aptamers selected against purified membrane proteins failed to recognize their targets in live cells. Cell-SELEX could develop aptamers against a particular target cell line to discriminate this cell line from others. Therefore, cell-SELEX has been widely used to select aptamers for the application of both diagnosis and therapy of various diseases, especially for cancer. In this review, the advantages and limitations of cell-SELEX and SELEX against purified protein will be compared. Various modified cell-SELEX techniques will be summarized, and application of cell-SELEX in cancer diagnosis and therapy will be discussed.
指数富集的配体系统进化技术（SELEX）是一个通过多轮重复的结合、分区和扩增，从组合单链寡核苷酸文库中逐步分离出高选择性单链DNA/RNA的过程。通过SELEX技术获得的单链DNA/RNA分子，即适配体，可针对多种靶标进行筛选，包括纯化的蛋白质、活细胞、组织、微生物、小分子等。在过去二十年中，随着SELEX技术的发展，出现了各种改良的SELEX方法。大多数适配体是通过传统的SELEX技术针对纯化的蛋白质筛选得到的。遗憾的是，越来越多的证据表明，针对纯化膜蛋白筛选得到的适配体无法在活细胞中识别其靶标。细胞SELEX技术能够针对特定的靶细胞系筛选适配体，从而将该细胞系与其他细胞系区分开来。因此，细胞SELEX技术已被广泛应用于筛选适配体，用于各种疾病尤其是癌症的诊断和治疗。在本综述中，将比较细胞SELEX技术与针对纯化蛋白质的SELEX技术的优缺点。将总结各种改良的细胞SELEX技术，并讨论细胞SELEX技术在癌症诊断和治疗中的应用。
