Bouilly Karine, Bonnard Marc, Gagnaire Béatrice, Renault Tristan, Lapègue Sylvie
IFREMER, Laboratoire de Génétique et Pathologie, 17390, La Tremblade, France.
Arch Environ Contam Toxicol. 2007 Jan;52(1):58-63. doi: 10.1007/s00244-005-0256-8. Epub 2006 Oct 26.
Diuron is a substituted urea herbicide used for agricultural and nonagricultural weed control. Its widespread use and relatively slow breakdown led us to analyze its influence on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in Pacific oysters, Crassostrea gigas. Adult oysters were subjected to two diuron concentrations (300 ng L(-1) and 3 microg L(-1)) for 11 weeks. Significantly higher aneuploidy level was observed in diuron-treated oysters compared with the control. Furthermore, the observed impact on aneuploidy persisted to the next generation as offspring exhibited significantly higher aneuploidy levels when their parents had been exposed to diuron. Significant increases in hemocyte parameters (cell mortality, phagocytosis, granulocyte percentage, reactive oxygen species, and lysosome presence) of the adults were also observed after 4 weeks of diuron exposure. The effects observed on oyster aneuploidy level and hemocyte parameters could have serious environmental and practical consequences.
敌草隆是一种用于农业和非农业杂草控制的取代脲类除草剂。其广泛使用以及相对缓慢的降解促使我们分析其对太平洋牡蛎（太平洋牡蛎）非整倍体水平（体细胞中染色体数降低的百分比）和血细胞参数的影响。将成年牡蛎暴露于两种敌草隆浓度（300 ng L(-1) 和3 μg L(-1)）下11周。与对照组相比，在经敌草隆处理的牡蛎中观察到显著更高的非整倍体水平。此外，对非整倍体的观察到的影响持续到下一代，因为当它们的父母暴露于敌草隆时，后代表现出显著更高的非整倍体水平。在敌草隆暴露4周后，还观察到成年牡蛎的血细胞参数（细胞死亡率、吞噬作用、粒细胞百分比、活性氧和溶酶体存在）显著增加。观察到的对牡蛎非整倍体水平和血细胞参数的影响可能会产生严重的环境和实际后果。
