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用于基于PCR的基因靶向的简化引物设计和微阵列引物数据库：裂殖酵母的两个网络工具

Simplified primer design for PCR-based gene targeting and microarray primer database: two web tools for fission yeast.

作者信息

Penkett Christopher J, Birtle Zoë E, Bähler Jürg

机构信息

Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Cambridge CB10 1HH, UK.

出版信息

Yeast. 2006 Oct 15;23(13):921-8. doi: 10.1002/yea.1422.

DOI:10.1002/yea.1422
PMID:17072893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2964512/
Abstract

PCR-based gene targeting is a popular method for manipulating yeast genes in their normal chromosomal locations. The manual design of primers, however, can be cumbersome and error-prone. We have developed a straightforward web-based tool that applies user-specified inputs to automate and simplify the task of primer selection for deletion, tagging and/or regulated expression of genes in Schizosaccharomyces pombe. This tool, named PPPP (for Pombe PCR Primer Programs), is available at http://www.sanger.ac.uk/PostGenomics/S_pombe/software/. We also present a searchable Microarray Primer Database to retrieve the sequences and accompanying information for primers and PCR products used to build our in-house Sz. pombe microarrays. This database contains information on both coding and intergenic regions to provide context for the microarray data, and it should be useful also for other applications, such as quantitative PCR. The database can be accessed at http://www.sanger.ac.uk/PostGenomics/S_pombe/microarray/.

摘要

基于聚合酶链反应（PCR）的基因靶向是一种在酵母基因的正常染色体位置进行操作的常用方法。然而，手动设计引物可能既繁琐又容易出错。我们开发了一个简单的基于网络的工具，该工具利用用户指定的输入来自动执行并简化为粟酒裂殖酵母中基因的缺失、标记和/或调控表达选择引物的任务。这个名为PPPP（粟酒裂殖酵母PCR引物程序）的工具可在http://www.sanger.ac.uk/PostGenomics/S_pombe/software/获取。我们还展示了一个可搜索的微阵列引物数据库，用于检索构建我们内部粟酒裂殖酵母微阵列所使用的引物和PCR产物的序列及相关信息。该数据库包含编码区和基因间区域的信息，可为微阵列数据提供背景，并且它对于其他应用（如定量PCR）也应该是有用的。该数据库可在http://www.sanger.ac.uk/PostGenomics/S_pombe/microarray/访问。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3c3/2964512/3641dc3ccc5e/yea0023-0921-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3c3/2964512/c4871ad7eeed/yea0023-0921-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3c3/2964512/064d1b39cdc0/yea0023-0921-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3c3/2964512/3641dc3ccc5e/yea0023-0921-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3c3/2964512/c4871ad7eeed/yea0023-0921-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3c3/2964512/064d1b39cdc0/yea0023-0921-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f3c3/2964512/3641dc3ccc5e/yea0023-0921-f3.jpg

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The fission yeast Rpb4 subunit of RNA polymerase II plays a specialized role in cell separation.
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A CRISPR/Cas9-based method and primer design tool for seamless genome editing in fission yeast.一种基于CRISPR/Cas9的用于裂殖酵母无缝基因组编辑的方法及引物设计工具。
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