川芎嗪对急性坏死性胰腺炎大鼠肠黏膜损伤的预防作用

Preventive effect of tetramethylpyrazine on intestinal mucosal injury in rats with acute necrotizing pancreatitis.

作者信息

Zhang Jian-Xin, Dang Sheng-Chun, Qu Jian-Guo, Wang Xue-Qing

机构信息

Department of General Surgery, the Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China.

出版信息

World J Gastroenterol. 2006 Oct 21;12(39):6386-90. doi: 10.3748/wjg.v12.i39.6386.

DOI:10.3748/wjg.v12.i39.6386

PMID:17072967

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4088152/

Abstract

AIM

To evaluate the role of microcirculatory disorder (MCD) and the therapeutic effectiveness of tetramethylpyrazine (TMP) on intestinal mucosa injury in rats with acute necrotizing pancreatitis (ANP).

METHODS

A total of 192 Sprague-Dawley rats were randomly divided into three groups: normal control group (C group), ANP group not treated with TMP (P group), ANP group treated with TMP (T group). An ANP model was induced by injection of 50 g/L sodium taurocholate under the pancreatic membrane (4 mL/kg). C group received isovolumetric injection of 9 g/L physiological saline solution using the same method. T group received injection of TMP (10 mL/kg) via portal vein. Radioactive biomicrosphere technique was used to measure the blood flow at 0.5, 2, 6 and 12 h after the induction of ANP. Samples of pancreas, distal ileum were collected to observe pathological changes using a validated histology score. Intestinal tissues were also used for examination of myeloperoxidase (MPO) expressed intracellularly in azurophilic granules of neutrophils.

RESULTS

The blood flow was significantly lower in P group than in C group (P < 0.01). The pathological changes were aggravated significantly in P group. The longer the time, the severer the pathological changes. The intestinal MPO activities were significantly higher in P group than in C group (P < 0.01). The blood flow of intestine was significantly higher in T group than in P group after 2 h (P < 0.01). The pathological changes were alleviated significantly in T group. MPO activities were significantly lower in T group than in P group (P < 0.01 or P < 0.05). There was a negative correlation between intestinal blood flow and MPO activity (r = -0.981, P < 0.01) as well as between intestinal blood flow and pathologic scores (r = -0.922, P < 0.05).

CONCLUSION

MCD is an important factor for intestinal injury in ANP. TMP can ameliorate the condition of MCD and the damage to pancreas and intestine.

摘要

目的

评估微循环障碍（MCD）在急性坏死性胰腺炎（ANP）大鼠肠黏膜损伤中的作用以及川芎嗪（TMP）对其的治疗效果。

方法

将192只Sprague-Dawley大鼠随机分为三组：正常对照组（C组）、未用TMP治疗的ANP组（P组）、用TMP治疗的ANP组（T组）。通过在胰膜下注射50 g/L牛磺胆酸钠（4 mL/kg）诱导ANP模型。C组采用相同方法等量注射9 g/L生理盐水溶液。T组通过门静脉注射TMP（10 mL/kg）。采用放射性生物微球技术在诱导ANP后0.5、2、6和12小时测量血流量。收集胰腺、回肠末端样本，使用经过验证的组织学评分观察病理变化。肠组织还用于检测中性粒细胞嗜天青颗粒中细胞内表达的髓过氧化物酶（MPO）。

结果

P组血流量显著低于C组（P < 0.01）。P组病理变化明显加重。时间越长，病理变化越严重。P组肠MPO活性显著高于C组（P < 0.01）。2小时后T组肠血流量显著高于P组（P < 0.01）。T组病理变化明显减轻。T组MPO活性显著低于P组（P < 0.01或P < 0.05）。肠血流量与MPO活性之间呈负相关（r = -0.981，P < 0.01），肠血流量与病理评分之间也呈负相关（r = -0.922，P < 0.05）。