Kim Soyoun, Kim Youngdeuk, Kim Philseok, Ha Jeongmin, Kim Kyunyoung, Sohn Mijin, Yoo Jin-San, Lee Jungeun, Kwon Jung-Ah, Lee Kap No
Chemistry Department, Dongguk University, Seoul, Korea.
Anal Chem. 2006 Nov 1;78(21):7392-6. doi: 10.1021/ac0520487.
Protein chips are a powerful emerging technology with extensive biomedical applications. However, the development of optimal, economical surface materials capable of maintaining the activity of embedded proteins is a challenge. Here, we introduce a new optimized, low-cost, sol-gel biomaterial for use in protein chips with femtogram-level sensitivity. A novel protein chip material with significantly improved physical properties and sensitivity was produced using unique screening and selection methods. Using this platform, the sensitive, specific detection of the interactions between an HIV antigen and its antibody and between a cyclin-kinase protein pair was observed. This study is the first to demonstrate the detection of protein-protein interactions on sol-gel microarrays and describes an important improvement in the physical properties of sol-gel-derived protein chip materials for biomedical research.
蛋白质芯片是一项具有广泛生物医学应用的强大新兴技术。然而，开发能够维持嵌入蛋白质活性的最佳、经济的表面材料是一项挑战。在此，我们介绍一种用于蛋白质芯片的新型优化、低成本的溶胶 - 凝胶生物材料，其具有飞克级灵敏度。使用独特的筛选和选择方法制备出了一种物理性质和灵敏度显著改善的新型蛋白质芯片材料。利用该平台，观察到了HIV抗原与其抗体之间以及细胞周期蛋白 - 激酶蛋白对之间相互作用的灵敏、特异性检测。本研究首次证明了在溶胶 - 凝胶微阵列上检测蛋白质 - 蛋白质相互作用，并描述了用于生物医学研究的溶胶 - 凝胶衍生蛋白质芯片材料在物理性质方面的一项重要改进。
