Kishimoto T, Fukuzawa Y, Tada M
Department of Environmental Medicine, Shimane Medical University, Izumo, Japan.
Int J Biometeorol. 1990 Dec;34(3):146-50. doi: 10.1007/BF01048711.
The combined effect of mercury (HgCl2) and high temperature on the growth and synthesis of nucleic acid and protein, and on the cell cycle of HeLa S3 cells was investigated. The subsequent growth of the cells was dose-dependently inhibited by mercury at 37.2 degrees and 41.2 degrees C. The inhibitory effect of mercury on subsequent growth was enhanced at the higher temperature. IC50 values for DNA and RNA synthesis but not protein synthesis, at 41.2 degrees C, were significantly lower than those at 37.2 degrees C (P less than 0.05, P less than 0.01, respectively). Flow cytometric analysis using synchronous cells indicated the possibility of blocking of cell cycle progression in the early part of S phase by the combined treatment. These results suggest that the cytotoxicity of mercury to cell growth was enhanced at the higher temperature and that this enhancement is related to the increased inhibitory effect of mercury on DNA and RNA synthesis and on the cell cycle at high temperatures.
研究了汞（HgCl₂）与高温对核酸和蛋白质生长与合成以及对HeLa S3细胞周期的联合作用。在37.2℃和41.2℃时，汞对细胞的后续生长呈剂量依赖性抑制。在较高温度下，汞对后续生长的抑制作用增强。在41.2℃时，DNA和RNA合成的IC50值（蛋白质合成的IC50值未出现此情况）显著低于37.2℃时的IC50值（分别为P＜0.05，P＜0.01）。使用同步化细胞进行的流式细胞术分析表明，联合处理可能在S期早期阻断细胞周期进程。这些结果表明，在较高温度下汞对细胞生长的细胞毒性增强，且这种增强与汞在高温下对DNA和RNA合成以及细胞周期的抑制作用增加有关。
