[DNA结合抑制因子4启动子甲基化状态在急性白血病中的意义]
[The significance of DNA-binding inhibitor 4 promoter methylation status in acute leukemia].
作者信息
Zhao Yu, Yu Li, Wang Quan-shun, Li Hong-hua, Bo Jian, Dou Li-ping
机构信息
Department of Hematology, General Hospital of PLA, Beijing 100853, China.
出版信息
Zhonghua Nei Ke Za Zhi. 2006 Jul;45(7):576-8.
OBJECTIVE
To investigate the relationship between promoter methylation of inhibitor of DNA binding 4 (Id4) gene and acute leukemia.
METHODS
We detected the status of promoter methylation of Id4 gene in peripheral blood, peripheral blood stem cell transplantation (PBSC) and bone marrow samples from health donors, bone marrow cells from leukemia patients and leukemia cell lines with MS-PCR methods.
RESULTS
The frequency of Id4 promoter methylation was 21/25 in acute myeloid leukemia (84%), 12/14 in acute lymphoblastic leukemia (86%), 8/8 in relapse acute leukemias and 2/2 in leukemia cell lines whereas no methylation was detected in peripheral blood, PBSC and bone marrow samples from healthy donors. In addition, Hek937 cell line was unmethylated.
CONCLUSION
Id4 promoter methylation was detected only in acute leukemia patients and leukemia cell lines and it might play a role in leukemia genesis.
目的
探讨DNA结合抑制因子4（Id4）基因启动子甲基化与急性白血病的关系。
方法
采用甲基化特异性聚合酶链反应（MS-PCR）方法检测健康供者外周血、外周血干细胞移植（PBSC）及骨髓标本、白血病患者骨髓细胞及白血病细胞系中Id4基因启动子甲基化状态。
结果
急性髓系白血病中Id4启动子甲基化频率为21/25（84%），急性淋巴细胞白血病中为12/14（86%），复发急性白血病中为8/8，白血病细胞系中为2/2，而健康供者外周血、PBSC及骨髓标本未检测到甲基化。此外，Hek937细胞系未甲基化。
结论
仅在急性白血病患者及白血病细胞系中检测到Id4启动子甲基化，其可能在白血病发生中起作用。
Zotero 插件