Liu Fei, Xu Rui-rong
Department of Hematology, Yantaishan Hospital, Shandong 264001.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Apr;32(4):471-3.
To investigate the correlation between Chinese medical syndrome type (CMST) and the ID4 gene promoter methylation state in the bone marrow cells of acute myeloid leukemia (AML) patients, and to discuss the correlation between ID4 gene methylation and the occurrence, development of AML.
Thirty-five inpatients or outpatients from the Department of Hematology, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine were recruited as the test group, while 10 healthy volunteers from the health medical center of the Affiliated Hospital, Shandong University of Traditional Chinese Medicine were recruited as the control group. The ID4 gene promoter methylation states were detected using methylation specific polymerase chain reaction (MS-PCR) in the two groups. Inter-group comparison was performed and CMSTs were compared to analyze the correlation between CMSTs and the gene promoter methylation.
Twenty-seven AML patients (77.1%) had methylation of ID4 gene, showing statistical difference when compared with the control group (P < 0.01). The ID4 methylation positive rate of ID4 gene promoter methylation was sequenced from low to high as qi and yin deficiency syndrome < inter-accumulation of blood stasis and phlegm syndrome < toxic heat inflaming syndrome, showing statistical difference when compared with the control group (P < 0.05). The peripheral white blood cells and the bone marrow blast cells were higher in ID4 methylation positive patients than in the ID4 methylation negative control patients with statistical difference (P < 0.05).
Patients of inter-accumulation of blood stasis and phlegm syndrome and toxic heat inflaming syndrome were more likely to have ID4 gene methylation. The ID4 methylation positive expression has verified the essence of evil domination in the early AML at the molecular level. It can also reflect the degree of malignancy of AML to some extent.
探讨急性髓系白血病（AML）患者中医证型与骨髓细胞中ID4基因启动子甲基化状态的相关性，以及ID4基因甲基化与AML发生、发展的关系。
选取山东中医药大学附属医院血液科35例住院或门诊患者作为试验组，选取山东中医药大学附属医院健康医学中心10例健康志愿者作为对照组。采用甲基化特异性聚合酶链反应（MS-PCR）检测两组ID4基因启动子甲基化状态。进行组间比较，并比较中医证型，分析中医证型与基因启动子甲基化的相关性。
27例AML患者（77.1%）存在ID4基因甲基化，与对照组比较差异有统计学意义（P<0.01）。ID4基因启动子甲基化的ID4甲基化阳性率由低到高依次为气阴两虚证<痰瘀互结证<热毒炽盛证，与对照组比较差异有统计学意义（P<0.05）。ID4甲基化阳性患者外周血白细胞及骨髓原始细胞高于ID4甲基化阴性的对照组患者，差异有统计学意义（P<0.05）。
痰瘀互结证和热毒炽盛证患者更易发生ID4基因甲基化。ID4甲基化阳性表达从分子水平验证了AML早期邪盛的本质，也能在一定程度上反映AML的恶性程度。
