Zhao Yu, Wang Quan-Shun, Li Hong-Hua, Jing Yu, Bo Jian, Wang Shu-Hong, Yu Li
Department of Hematology, The General Hospital of PLA, Beijing 100853, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Dec;16(6):1418-20.
This study was purposed to investigate the status of Id4 gene promoter methylation in malignant hematologic cell lines. MS-PCR methods were used to detect the status of Id4 gene methylation in K562, HL-60, Ramous and CA46. Meanwhile bone marrow cells in healthy individuals and Hek937, a benign renal cell line, were involved as control. The results showed that Id4 gene kept unmethylated in bone marrow cells from healthy individuals and Hek937 cells, while Id4 gene was methylated in all of K562, HL-60, Ramous and CA46 cell lines. It is concluded that different from healthy bone marrow cells and non-malignant cell line Hek937, Id4 gene was methylated in leukemia and lymphoma cell lines including K562, HL-60, Ramous and CA46. The change of Id4 gene methylation is thought to be associated with occurrence of hematologic malignancies.
本研究旨在探讨Id4基因启动子甲基化在恶性血液学细胞系中的状态。采用甲基化特异性聚合酶链反应（MS-PCR）方法检测K562、HL-60、Ramous和CA46细胞系中Id4基因的甲基化状态。同时，选取健康个体的骨髓细胞和良性肾细胞系Hek937作为对照。结果显示，健康个体的骨髓细胞和Hek937细胞中Id4基因保持未甲基化状态，而在所有K562、HL-60、Ramous和CA46细胞系中Id4基因均发生甲基化。结论：与健康骨髓细胞和非恶性细胞系Hek937不同，Id4基因在包括K562、HL-60、Ramous和CA46在内的白血病和淋巴瘤细胞系中发生甲基化。Id4基因甲基化的改变被认为与血液系统恶性肿瘤的发生有关。
