Collins L V, Hackett J
Department of Microbiology, University of Adelaide, South Australia.
J Bacteriol. 1991 Apr;173(8):2521-9. doi: 10.1128/jb.173.8.2521-2529.1991.
The rfc gene of Salmonella typhimurium was located in a 1.75-kb HindIII fragment and restored wild-type lipopolysaccharide synthesis ability to both an older rfc point mutant and new rfc::IS10 mutants. DNA sequencing of the HindIII fragment revealed an open reading frame which could encode a protein of 407 amino acids with an Mr of 47,472 and also revealed potential translation signals. Modulator codons accounted for 12.5% of the total codon content, providing a possible explanation for the nondetectability of the protein in subcellular systems. Secondary structure analysis suggested the presence of transmembrane beta-sheet structures, implying a possible role for the protein in translocation of hydrophilic O-antigen-containing materials. Salmonella strains of groups A, B, and D1 contained rfc-homologous DNA, but strains of groups C1, C2, C3, D2, and E2 did not.
鼠伤寒沙门氏菌的rfc基因位于一个1.75kb的HindIII片段中，它将野生型脂多糖合成能力恢复给了一个较老的rfc点突变体和新的rfc::IS10突变体。对HindIII片段进行DNA测序，发现了一个开放阅读框，它可以编码一个由407个氨基酸组成、Mr为47472的蛋白质，同时也发现了潜在的翻译信号。调节子密码子占总密码子含量的12.5%，这可能是该蛋白在亚细胞系统中无法检测到的原因。二级结构分析表明存在跨膜β-折叠结构，这意味着该蛋白可能在含亲水性O抗原物质的转运中发挥作用。A、B和D1组的沙门氏菌菌株含有rfc同源DNA，但C1、C2、C3、D2和E2组的菌株则没有。
