• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用克隆在质粒上的无活性基因替换大肠杆菌的fip基因。

Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid.

作者信息

Russel M, Model P

出版信息

J Bacteriol. 1984 Sep;159(3):1034-9. doi: 10.1128/jb.159.3.1034-1039.1984.

DOI:10.1128/jb.159.3.1034-1039.1984
PMID:6384177
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215764/
Abstract

To determine whether the fip gene of Escherichia coli, which is required for filamentous phage assembly, is required for cell viability, we replaced the chromosomal copy of the gene with an inactive copy introduced on a plasmid. We found that the fip gene is dispensable. The method we devised, which should be generally useful, was also tested with an inactivated rho gene. As expected, the rho gene is essential.

摘要

为了确定大肠杆菌中丝状噬菌体组装所需的fip基因是否为细胞生存力所必需，我们用质粒上引入的无活性拷贝替换了该基因的染色体拷贝。我们发现fip基因是可有可无的。我们设计的方法应具有普遍实用性，还用失活的rho基因进行了测试。正如预期的那样，rho基因是必不可少的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e9c/215764/ba7bf0000564/jbacter00232-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e9c/215764/54b37bc59e70/jbacter00232-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e9c/215764/ba7bf0000564/jbacter00232-0236-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e9c/215764/54b37bc59e70/jbacter00232-0235-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e9c/215764/ba7bf0000564/jbacter00232-0236-a.jpg

相似文献

1
Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid.用克隆在质粒上的无活性基因替换大肠杆菌的fip基因。
J Bacteriol. 1984 Sep;159(3):1034-9. doi: 10.1128/jb.159.3.1034-1039.1984.
2
Characterization of the cloned fip gene and its product.克隆的fip基因及其产物的特性分析。
J Bacteriol. 1984 Feb;157(2):526-32. doi: 10.1128/jb.157.2.526-532.1984.
3
In vivo transfer of chromosomal mutations onto multicopy plasmids by transduction with bacteriophage P1.通过噬菌体P1转导将染色体突变体内转移到多拷贝质粒上。
Gene. 1985;40(2-3):241-6. doi: 10.1016/0378-1119(85)90046-0.
4
Site-directed insertion mutagenesis with cloned fragments in Escherichia coli by P1 phage transduction.利用P1噬菌体转导在大肠杆菌中对克隆片段进行定点插入诱变。
Mol Gen Genet. 1990 Jan;220(2):339-40. doi: 10.1007/BF00260506.
5
High frequency generalized transduction by miniMu plasmid phage.微小 Mu 质粒噬菌体介导的高频广义转导
Genetics. 1987 Jun;116(2):201-6. doi: 10.1093/genetics/116.2.201.
6
[Cloning of the gpp gene of Escherichia coli and the use of recBC, sbcB cells for inserting its mutant allele into the chromosomal structure].[大肠杆菌gpp基因的克隆以及利用recBC、sbcB细胞将其突变等位基因插入染色体结构]
Genetika. 1988 Aug;24(8):1333-42.
7
Coliphage P1-mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: use for complementation and recombinational analyses.噬菌体温和噬菌体P1介导的克隆DNA从大肠杆菌到黄色粘球菌的转导：用于互补和重组分析。
J Bacteriol. 1983 Jul;155(1):317-29. doi: 10.1128/jb.155.1.317-329.1983.
8
[Increase in stability of the recombinant phage M13 carrying Escherichia coli genes rpIJL by reducing expression of cloned genes].[通过降低克隆基因的表达提高携带大肠杆菌基因rpIJL的重组噬菌体M13的稳定性]
Genetika. 1990 Mar;26(3):557-9.
9
Cloning and characterization of an Escherichia coli gene, pcnB, affecting plasmid copy number.影响质粒拷贝数的大肠杆菌基因pcnB的克隆与特性分析
Mol Microbiol. 1989 Jul;3(7):903-10. doi: 10.1111/j.1365-2958.1989.tb00239.x.
10
Efficient introduction of cloned mutant alleles into the Escherichia coli chromosome.将克隆的突变等位基因高效导入大肠杆菌染色体。
J Bacteriol. 1991 Apr;173(8):2633-8. doi: 10.1128/jb.173.8.2633-2638.1991.

引用本文的文献

1
Analysis of the link between the redox state and enzymatic activity of the HtrA (DegP) protein from Escherichia coli.大肠杆菌HtrA（DegP）蛋白氧化还原状态与酶活性之间的联系分析。
PLoS One. 2015 Feb 24;10(2):e0117413. doi: 10.1371/journal.pone.0117413. eCollection 2015.
2
The CoxD protein of Oligotropha carboxidovorans is a predicted AAA+ ATPase chaperone involved in the biogenesis of the CO dehydrogenase [CuSMoO2] cluster.嗜羧寡养菌的CoxD蛋白是一种预测的AAA+ATP酶伴侣蛋白，参与一氧化碳脱氢酶[CuSMoO2]簇的生物合成。
J Biol Chem. 2009 Apr 3;284(14):9578-86. doi: 10.1074/jbc.M805354200. Epub 2009 Feb 2.
3

本文引用的文献

1
A bacteriophage containing RNA.一种含有RNA的噬菌体。
Proc Natl Acad Sci U S A. 1961 Mar 15;47(3):282-9. doi: 10.1073/pnas.47.3.282.
2
The B66.0 protein of Escherichia coli is the product of the dnaK+ gene.大肠杆菌的B66.0蛋白是dnaK⁺基因的产物。
J Bacteriol. 1982 Mar;149(3):1175-7. doi: 10.1128/jb.149.3.1175-1177.1982.
3
Identification of a novel genetic element in Escherichia coli K-12.在大肠杆菌K-12中鉴定一种新型遗传元件。
The extracellular death factor: physiological and genetic factors influencing its production and response in Escherichia coli.
细胞外死亡因子：影响其在大肠杆菌中产生及应答的生理和遗传因素
J Bacteriol. 2008 May;190(9):3169-75. doi: 10.1128/JB.01918-07. Epub 2008 Feb 29.
4
Escherichia coli mazEF-mediated cell death as a defense mechanism that inhibits the spread of phage P1.大肠杆菌mazEF介导的细胞死亡作为一种防御机制，可抑制噬菌体P1的传播。
Mol Genet Genomics. 2004 Sep;272(2):227-34. doi: 10.1007/s00438-004-1048-y. Epub 2004 Aug 14.
5
Escherichia coli mazEF-mediated cell death is triggered by various stressful conditions.大肠杆菌mazEF介导的细胞死亡由多种应激条件触发。
J Bacteriol. 2004 Jun;186(11):3663-9. doi: 10.1128/JB.186.11.3663-3669.2004.
6
Minimal and contributing sequence determinants of the cis-acting locus of transfer (clt) of streptomycete plasmid pIJ101 occur within an intrinsically curved plasmid region.链霉菌质粒pIJ101的顺式作用转移位点（clt）的最小和辅助序列决定因素存在于一个固有弯曲的质粒区域内。
J Bacteriol. 2000 Dec;182(23):6834-41. doi: 10.1128/JB.182.23.6834-6841.2000.
7
Immunolocalization of a cysteine protease in vacuoles, vesicles, and symbiosomes of pea nodule cells.豌豆根瘤细胞液泡、囊泡和共生体中半胱氨酸蛋白酶的免疫定位
Plant Physiol. 2000 Jun;123(2):521-30. doi: 10.1104/pp.123.2.521.
8
An additional regulatory gene for actinorhodin production in Streptomyces lividans involves a LysR-type transcriptional regulator.在天蓝色链霉菌中，另一个参与放线紫红素产生的调控基因涉及一个 LysR 型转录调节因子。
J Bacteriol. 1999 Jul;181(14):4353-64. doi: 10.1128/JB.181.14.4353-4364.1999.
9
Complete nucleotide sequence of the 27-kilobase virulence related locus (vrl) of Dichelobacter nodosus: evidence for extrachromosomal origin.结节拟杆菌27千碱基毒力相关基因座（vrl）的完整核苷酸序列：染色体外起源的证据
Infect Immun. 1999 Mar;67(3):1277-86. doi: 10.1128/IAI.67.3.1277-1286.1999.
10
Bacteriophage T4 gp2 interferes with cell viability and with bacteriophage lambda Red recombination.噬菌体T4 gp2会干扰细胞活力以及噬菌体λ Red重组。
J Bacteriol. 1999 Feb;181(4):1352-5. doi: 10.1128/JB.181.4.1352-1355.1999.
J Bacteriol. 1980 Oct;144(1):312-21. doi: 10.1128/jb.144.1.312-321.1980.
4
Establishment of Escherichia coli cells with an integrated high copy number plasmid.构建携带整合型高拷贝质粒的大肠杆菌细胞。
Mol Gen Genet. 1980;178(3):525-33. doi: 10.1007/BF00337857.
5
Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
6
A bacterial gene, fip, required for filamentous bacteriophage fl assembly.丝状噬菌体fl组装所需的细菌基因fip。
J Bacteriol. 1983 Jun;154(3):1064-76. doi: 10.1128/jb.154.3.1064-1076.1983.
7
Characterization of the cloned fip gene and its product.克隆的fip基因及其产物的特性分析。
J Bacteriol. 1984 Feb;157(2):526-32. doi: 10.1128/jb.157.2.526-532.1984.
8
New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase.新型多功能质粒载体，用于表达由与编码β-半乳糖苷酶酶活性羧基末端部分的lacZ基因序列融合的克隆基因所编码的杂交蛋白。
Gene. 1983 Nov;25(1):71-82. doi: 10.1016/0378-1119(83)90169-5.
9
Replacement and amplification of bacterial genes with sequences altered in vitro.用体外改变的序列替换和扩增细菌基因。
Proc Natl Acad Sci U S A. 1983 Aug;80(16):4894-8. doi: 10.1073/pnas.80.16.4894.
10
Coliphage P1-mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: use for complementation and recombinational analyses.噬菌体温和噬菌体P1介导的克隆DNA从大肠杆菌到黄色粘球菌的转导：用于互补和重组分析。
J Bacteriol. 1983 Jul;155(1):317-29. doi: 10.1128/jb.155.1.317-329.1983.