Chen Lei, Lagunoff Michael
Department of Microbiology, University of Washington, 1959 N.E. Pacific Street, Box 357242, Seattle, WA 98195, USA.
Virology. 2007 Mar 15;359(2):425-35. doi: 10.1016/j.virol.2006.09.044. Epub 2006 Oct 30.
Kaposi's Sarcoma-associated herpesvirus encodes a homolog of the human cellular interleukin-6 that may play a formative role in many KSHV-related diseases. While the viral IL-6 can signal similarly to its human counterpart little is known about the role of vIL-6 during KSHV infection. Using homologous recombination and selection in eukaryotic cells, a KSHV isolate was purified that does not express vIL-6 as was a control recombinant that left vIL-6 intact. The two viruses establish and maintain latency to similar levels in BJAB B-cells, reactivate to similar levels in B-cells and Monkey kidney cells and have very similar KSHV gene expression patterns. BJAB cells expressing KSHV survive better than the parental BJAB cells in low serum and the vIL-6 deletion does not abrogate this growth advantage. Thus vIL-6 is not essential for establishment, maintenance, or reactivation from latency in cell culture and is not involved in the survival of infected BJAB B-cells in low serum.
卡波西肉瘤相关疱疹病毒编码一种人类细胞白细胞介素-6的同源物，它可能在许多与卡波西肉瘤相关疱疹病毒有关的疾病中发挥重要作用。虽然病毒白细胞介素-6的信号传导方式与其人类对应物相似，但关于病毒白细胞介素-6在卡波西肉瘤相关疱疹病毒感染过程中的作用却知之甚少。利用真核细胞中的同源重组和筛选技术，纯化出一种不表达病毒白细胞介素-6的卡波西肉瘤相关疱疹病毒分离株，并将保留完整病毒白细胞介素-6的重组体作为对照。这两种病毒在BJAB B细胞中建立和维持潜伏状态的水平相似，在B细胞和猴肾细胞中重新激活的水平相似，并且具有非常相似的卡波西肉瘤相关疱疹病毒基因表达模式。在低血清条件下，表达卡波西肉瘤相关疱疹病毒的BJAB细胞比亲代BJAB细胞存活得更好，而病毒白细胞介素-6的缺失并没有消除这种生长优势。因此，病毒白细胞介素-6对于细胞培养中潜伏状态的建立、维持或重新激活并非必不可少，并且不参与感染的BJAB B细胞在低血清中的存活。
