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用于测定传染性人类疱疹病毒 8 滴度的 50%组织培养感染剂量测定。

Fifty percent tissue culture infective dose assay for determining the titer of infectious human herpesvirus 8.

机构信息

Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

出版信息

J Clin Microbiol. 2013 Jun;51(6):1931-4. doi: 10.1128/JCM.00761-13. Epub 2013 Apr 3.

DOI:10.1128/JCM.00761-13
PMID:23554189
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3716109/
Abstract

We have developed a human herpesvirus 8 (HHV-8) 50% tissue culture infective dose (TCID50) assay using the T1H6-DC-SIGN cell line. Infection of T1H6-DC-SIGN cells with HHV-8 induces expression of β-galactosidase, which was used to determine TCID50 levels. Validation of TCID50 values was performed by immunofluorescence assay of HHV-8 infection of immature dendritic cells at various TCID50 doses.

摘要

我们使用 T1H6-DC-SIGN 细胞系开发了一种人类疱疹病毒 8 型 (HHV-8) 的 50%组织培养感染剂量 (TCID50) 测定法。HHV-8 感染 T1H6-DC-SIGN 细胞会诱导β-半乳糖苷酶的表达，该酶可用于确定 TCID50 水平。通过在不同 TCID50 剂量下对不成熟树突状细胞的 HHV-8 感染进行免疫荧光检测，验证了 TCID50 值。

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J Clin Microbiol. 2013 Jun;51(6):1931-4. doi: 10.1128/JCM.00761-13. Epub 2013 Apr 3.
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