Konttinen Y T, Rees R, Hukkanen M, Grönblad M, Tolvanen E, Gibson S J, Polak J M, Brewerton D A
Fourth Department of Medicine, University of Helsinki, Central Hospital, Finland.
J Rheumatol. 1990 Dec;17(12):1586-91.
Previous evidence has been presented that neurogenic input may influence adjuvant induced arthritis (AA) in rats. We now present evidence of alterations in synovial nerves in AA. Nerves were studied in well perfused and fixed rats, using immunohistochemistry with the sensitive avidin-biotin peroxidase complex (ABC) method and heterologous antisera to cytoskeletal protein gene product 9.5 (PGP) and the neuropeptides substance P and calcitonin gene related peptide (CGRP). The innervation of synovium was compared in normal rats and rats with AA. Observations concordant with what has been reported for neuropeptide nerves in the synovium of patients with rheumatoid arthritis (RA) are presented. It has been suggested that neural peptide substances are reduced in nerves of synovium from patients with RA. In the AA rat a specific reduction of lining zone and sublining nerves in the synovium was noted. The AA rat model is very suitable for studying the involvement of synovial nerves in arthritis, permitting optimal preservation of immunoreactive neural epitopes.
先前已有证据表明，神经源性输入可能会影响大鼠的佐剂性关节炎（AA）。我们现在展示了AA中滑膜神经改变的证据。使用敏感的抗生物素蛋白-生物素过氧化物酶复合物（ABC）方法以及针对细胞骨架蛋白基因产物9.5（PGP）和神经肽P物质及降钙素基因相关肽（CGRP）的异源抗血清，在灌注良好且固定的大鼠中研究神经。比较了正常大鼠和患有AA的大鼠滑膜的神经支配情况。呈现了与类风湿性关节炎（RA）患者滑膜中神经肽神经的报道一致的观察结果。有人提出，RA患者滑膜神经中的神经肽物质会减少。在AA大鼠中，注意到滑膜内衬区和滑膜下层神经有特异性减少。AA大鼠模型非常适合研究滑膜神经在关节炎中的作用，能够最佳地保留免疫反应性神经表位。
