Hukkanen M, Grönblad M, Rees R, Kottinen Y T, Gibson S J, Hietanen J, Polak J M, Brewerton D A
Fourth Department of Medicine, University of Helsinki, Finland.
J Rheumatol. 1991 Feb;18(2):177-83.
Simultaneous visualization of nerves and mast cells in the rat synovium was possible with double staining. Thus, a direct comparison could be made of nerves and mast cells in the ankle joints of healthy rats and in those with severe adjuvant induced polyarthritis. Nerves were studied with avidin-biotin-peroxidase complex (ABC) immunostaining, using heterologous antisera to protein gene product 9.5 (PGP 9.5), a recently discovered neural protein, and the neuropeptides substance P and calcitonin gene related peptide (CGRP). Mast cells were visualized by metachromatic staining of granule heparin. With double staining of sections, a parallel distribution of mast cells and nerves in all parts of the normal synovium was noted. In rats with adjuvant induced arthritis, a near total parallel disappearance of mast cells and nerves in the synovium occurred. In the arthritic rat such mast cell/nerve "units" were only present in the region where synovium attaches to bone. The observed regional depletion of both nerves and mast cells in arthritis may be of importance in the pathophysiology of arthritis.
通过双重染色可以同时观察大鼠滑膜中的神经和肥大细胞。因此，可以直接比较健康大鼠和严重佐剂诱导性多关节炎大鼠踝关节中的神经和肥大细胞。使用抗蛋白质基因产物9.5（PGP 9.5，一种最近发现的神经蛋白）的异源抗血清以及神经肽P物质和降钙素基因相关肽（CGRP），通过抗生物素蛋白-生物素-过氧化物酶复合物（ABC）免疫染色研究神经。通过颗粒肝素的异染性染色观察肥大细胞。通过对切片进行双重染色，发现正常滑膜各部位的肥大细胞和神经呈平行分布。在佐剂诱导性关节炎大鼠中，滑膜中的肥大细胞和神经几乎完全平行消失。在患关节炎的大鼠中，这种肥大细胞/神经“单位”仅存在于滑膜与骨附着的区域。在关节炎中观察到的神经和肥大细胞的区域性耗竭可能在关节炎的病理生理学中具有重要意义。
