Pereira da Silva J A, Carmo-Fonseca M
Instituto de Histologia e Embriologia, Faculty of Medicine, University of Libson, Portugal.
J Rheumatol. 1990 Dec;17(12):1592-9.
The innervation of normal and rheumatoid human synovium was studied by immunofluorescence microscopy. Antibodies against the general neuronal marker protein gene product (PGP) 9.5 and specific neuropeptides were used. We observed sensory nerves containing substance P (SP) and calcitonin gene related peptide (CGRP) as well as autonomic sympathetic fibers immunoreactive for neuropeptide tyrosine (NPY), its C terminal peptide (C-PON) and the catecholamine synthesizing enzyme tyrosine hydroxylase (TH). Three subpopulations of nerve fibers labelled with SP and CGRP were identified: some stained for SP or CGRP only and others contained both peptides. NPY/C-PON and TH labelled predominantly perivascular nerves. Quantification of immunostained nerves revealed a significantly decreased innervation of rheumatoid synovia. The densities of both PGP 9.5 and neuropeptide containing nerves were lower in all rheumatoid samples. Our results are compatible with a local release of neuropeptides into joint fluid and point to a disturbed neuronal control of rheumatoid synovial tissue.
通过免疫荧光显微镜对正常和类风湿性人类滑膜的神经支配进行了研究。使用了针对一般神经元标志物蛋白基因产物（PGP）9.5和特定神经肽的抗体。我们观察到含有P物质（SP）和降钙素基因相关肽（CGRP）的感觉神经，以及对神经肽酪氨酸（NPY）、其C末端肽（C-PON）和儿茶酚胺合成酶酪氨酸羟化酶（TH）具有免疫反应性的自主交感纤维。鉴定出了用SP和CGRP标记的三个神经纤维亚群：一些仅对SP或CGRP染色，另一些则同时含有这两种肽。NPY/C-PON和TH主要标记血管周围神经。对免疫染色神经的定量分析显示类风湿性滑膜的神经支配明显减少。在所有类风湿性样本中，PGP 9.5和含神经肽神经的密度均较低。我们的结果与神经肽向关节液中的局部释放相一致，并表明类风湿性滑膜组织的神经元控制受到干扰。
