Folcòn J, Thibault C, Blazquez J L, Vaudry H, Ling N, Colin J P
Laboratoire de Biologie Cellulaire, URA CNRS 290, Université de Poitiers, France.
Pflugers Arch. 1990 Oct;417(2):243-5. doi: 10.1007/BF00370707.
Atrial natriuretic factor (ANF) stimulates accumulation of cyclic GMP in a photosensitive organ, as evidenced for the first time in cultured trout pineals. Stimulation was rapid (within a few min), dose-dependent, and stronger in organs cultured in darkness than in those cultured under light. After 30 min in the dark, (i) cyclic AMP levels were slightly increased at 10(-7) mole/l of ANF, (ii) cyclic GMP and cyclic AMP increased dramatically after inhibition of the phosphodiesterases by isobutylmethylxanthine (IBMX), (iii) ANF and IBMX effects were more than additive on cyclic GMP, (iv) pertussis toxin decreased the cyclic GMP response to ANF. These responses were affected by light. The possibility that cyclic GMP might be a second messenger of both light and chemical (ANF) inputs, in pineal photoreceptor cells, is hypothetized.
心房利钠因子（ANF）可刺激一个感光器官中环状鸟苷酸（cGMP）的积累，这首次在培养的虹鳟松果体中得到证实。刺激迅速（几分钟内），呈剂量依赖性，且在黑暗中培养的器官中比在光照下培养的器官中更强。在黑暗中30分钟后，（i）在10⁻⁷摩尔/升的ANF作用下，环状腺苷酸（cAMP）水平略有升高，（ii）在异丁基甲基黄嘌呤（IBMX）抑制磷酸二酯酶后，cGMP和cAMP显著增加，（iii）ANF和IBMX对cGMP的作用大于相加效应，（iv）百日咳毒素降低了对ANF的cGMP反应。这些反应受光照影响。据此推测，在松果体光感受器细胞中，cGMP可能是光和化学（ANF）输入两者的第二信使。
