Nagata Keiko, Hide Michihiro, Tanaka Toshihiko, Ishii Kaori, Izawa Masao, Sairenji Takeshi, Tomita Katsuyuki, Shimizu Eiji
Division of Medical Oncology and Molecular Respirology, Department of Multidisciplinary Internal Medicine, Faculty of Medicine, Tottori University, Yonago, Tottori, Japan.
Allergol Int. 2006 Jun;55(2):181-4. doi: 10.2332/allergolint.55.181.
Since the first report of a dog that developed severe systemic symptoms in response to a second injection of sea anemone toxin by Richet and Portier in 1902, no clear human cases of anaphylaxis related to exposure to sea anemones has been reported in the literature.
A 24-year-old man with an episode of local urticaria on his first contact with a sea anemone (Stichodactyla haddoni), developed dyspnea, severe urticaria and hypotension on exposure to water containing the dead bodies of the organism. To study whether this reaction was mediated by antigen-specific IgE, we performed a histamine release test with blood, Western blotting with serum and lymphocyte proliferating test with peripheral blood mononuclear cells of the patient, for the homogenate of sea anemones.
The homogenate of sea anemones induced histamine release from the blood of the patient, but it also induced histamine release from the blood of control subjects. Moreover, it also caused hemolysis of blood of all donors. However, Western-blotting demonstrated the presence of an 86 kd protein-specific IgE in the serum of the patient.
Protein antigen(s) in sea anemones may cause anaphylactic shock under the influence of the cytolytic effects and/or lymphocyte-stimulating activity elicited by the toxin of sea anemones.
自1902年里歇和波捷首次报道一只狗在第二次注射海葵毒素后出现严重全身症状以来，文献中尚未报道明确的与接触海葵相关的人类过敏反应病例。
一名24岁男性在首次接触海葵（哈氏列指海葵）时出现局部荨麻疹，在接触含有该生物体尸体的水后出现呼吸困难、严重荨麻疹和低血压。为研究该反应是否由抗原特异性IgE介导，我们对患者的血液进行了组胺释放试验，用血清进行了蛋白质印迹分析，并用患者外周血单个核细胞对海葵匀浆进行了淋巴细胞增殖试验。
海葵匀浆可诱导患者血液释放组胺，但也可诱导对照受试者血液释放组胺。此外，它还导致所有供血者的血液发生溶血。然而，蛋白质印迹分析显示患者血清中存在86kd蛋白特异性IgE。
海葵中的蛋白质抗原可能在海葵毒素引起的细胞溶解作用和/或淋巴细胞刺激活性的影响下导致过敏性休克。
