Uszyński Waldemar, Zekanowska Ewa, Uszyński Mieczysław, Rystok Danuta
Oddział Połozniczo-Ginekologiczny Wojewódzkiego Szpitala we Włocławku.
Ginekol Pol. 2006 Aug;77(8):603-9.
THE BACKGROUND: It is believed that annexin V, an anticoagulant protein abundant in trophoblast, prevents circulating blood in the intervillous space from hypercoagulability and fibrin deposition. Distribution and the role of annexin V localized in other gestational tissues like myometrium and fetal membranes, as well as in amniotic fluid is unknown. The aim of this study was to determine the concentration of annexin V in the tissues under consideration.
The study group consisted of 40 healthy women at the 1st stage of labour, 16 of whom delivered by cesarean section. The strips of myometrium, placenta and fetal membranes, as well as the samples of amniotic fluid and the venous blood, were collected during surgery. Homogenates were prepared from the tissues. Annexin V was measured by immunoenzymatic method (ELISA).
Concentration of annexin V in the placenta was 122.65 +/- 33,14 ng/mg protein, in fetal membranes 136.31 +/- 49.30 ng/mg protein and in myometrium 65.40 +/- 30.72 ng/mg protein. There was a statistical difference between the concentrations in placenta and fetal membranes vs. myometrium (p<0.05). In amniotic fluid annexin V was found to be in low concentration (4.46 +/- 2.59 ng/ml, i.e., 1.60 +/- 1.21 ng/mg protein) while in blood plasma it was extremely low, over 6000 times lower than in placenta.
Annexin V is present not only in placenta, but also in fetal membranes and myometrium. Its concentration in fetal membranes is similar to that in placenta, while in myometrium it is 50% lower. Annexin V found in newly discovered sites probably plays a similar role like placental annexin V.
背景：据信，膜联蛋白V是一种在滋养层中丰富的抗凝蛋白，可防止绒毛间隙中的循环血液发生高凝和纤维蛋白沉积。膜联蛋白V在其他妊娠组织（如子宫肌层和胎膜）以及羊水中的分布和作用尚不清楚。本研究的目的是确定所研究组织中膜联蛋白V的浓度。
研究组由40名处于第一产程的健康女性组成，其中16人通过剖宫产分娩。在手术过程中收集子宫肌层、胎盘和胎膜条带，以及羊水和静脉血样本。从组织中制备匀浆。通过免疫酶法（ELISA）测量膜联蛋白V。
胎盘中膜联蛋白V的浓度为122.65±33.14 ng/mg蛋白，胎膜中为136.31±49.30 ng/mg蛋白，子宫肌层中为65.40±30.72 ng/mg蛋白。胎盘和胎膜与子宫肌层中的浓度存在统计学差异（p<0.05）。羊水中膜联蛋白V浓度较低（4.46±2.59 ng/ml，即1.60±1.21 ng/mg蛋白），而血浆中极低，比胎盘中低6000倍以上。
膜联蛋白V不仅存在于胎盘中，也存在于胎膜和子宫肌层中。其在胎膜中的浓度与胎盘中相似，而在子宫肌层中低50%。在新发现部位发现的膜联蛋白V可能发挥与胎盘膜联蛋白V类似的作用。
