Gunn L, Hardiman P, Tharmaratnam S, Lowe D, Chard T
Department of Obstetrics, Gynaecology, St Bartholomew's Hospital Medical College, London, UK.
Reprod Fertil Dev. 1996;8(7):1069-73. doi: 10.1071/rd9961069.
The concentrations of interleukin-1 alpha (IL-1 alpha) and IL-6 in pregnancy-associated tissues were investigated in term labour and delivery in the absence of labour (elective Caesarean section). Samples of amniotic fluid, placenta, fetal membranes, umbilical venous and, where possible, umbilical arterial blood were collected at delivery (37-41 weeks of gestation). Maternal blood was sampled during labour. Fluid and tissue extracts were assayed for IL-1 alpha and IL-6 by radioimmunoassay. Placenta and membranes were examined histologically for evidence of infection. Concentrations of IL-1 alpha and IL-6 in amniotic fluid and membrane extract, and IL-1 alpha in maternal and fetal blood, were raised after the onset of labour. Concentrations of both cytokines in the placenta remained unchanged. There was a good correlation between concentrations of both cytokines in amniotic fluid and membranes. There was also a significant correlation between concentrations of IL-1 alpha and IL-6 in amniotic fluid, placenta and membranes. It is suggested that the fetal membranes or maternal decidua, but not the placenta, internal fetal or maternal tissues, are the main sources of IL-1 alpha and IL-6 during labour.
在足月分娩和未临产（择期剖宫产）时，研究了妊娠相关组织中白细胞介素-1α（IL-1α）和IL-6的浓度。在分娩时（妊娠37 - 41周）采集羊水、胎盘、胎膜、脐静脉血样本，如有可能还采集脐动脉血样本。在分娩过程中采集母体血液样本。通过放射免疫分析法检测液体和组织提取物中的IL-1α和IL-6。对胎盘和胎膜进行组织学检查以寻找感染证据。临产开始后，羊水和胎膜提取物中IL-1α和IL-6的浓度以及母体和胎儿血液中IL-1α的浓度升高。胎盘中两种细胞因子的浓度保持不变。羊水和胎膜中两种细胞因子的浓度之间存在良好的相关性。羊水、胎盘和胎膜中IL-1α和IL-6的浓度之间也存在显著相关性。提示胎膜或母体蜕膜而非胎盘、胎儿内部或母体组织是分娩期间IL-1α和IL-6的主要来源。
