Wu Hui-Qin, Huang Xiao-Lan, Lin Xiao-Shan, Huang Fang, Ge Fa-Huan
Guangdong Key Laboratory of Chemical Emergency Test, China National Analytical Center, Guangzhou 510070, China.
Zhong Yao Cai. 2006 Aug;29(8):788-92.
To establish the fingerprint chromatogram of Amomun kravak Pierre ex Gagnep.
The composition of the essential oil was isolated by PR solvent and determined by GC-MS. Forty-two species of compounds were identified, of which the relative contents were calculated using square peaks to normalization, among which the character components were 1,8-cineole, beta-pinene, alpha-pinene, p-cymene, etc. EMIC software was used and EMIC fingerprint chromatogram was established by selecting the character ion of those 10 compounds. Different extraction methods were also compared.
The GC-MS fingerprint chromatogram can provide qualitative information confirming compounds of the corresponding chromatographic peaks. EMIC was first used to reason the quality control of the medicine quality and it can establish the foundation for safe and effective quality control of the medicine and its product. PR solvent can easily extract the essential oil from the medicine.
This method has the advantage of high efficiency and low cost, and the GC-MS fingerprint chromatogram provided the ascription and quality of the medicine and will play an important role in promoting the modernization of Chinese traditional medicine.
建立白豆蔻的指纹图谱。
采用PR溶剂分离挥发油成分，用气相色谱-质谱联用仪（GC-MS）测定。共鉴定出42种化合物，采用面积归一化法计算各成分的相对含量，其中特征性成分有1,8-桉叶素、β-蒎烯、α-蒎烯、对伞花烃等。利用EMIC软件，选择这10种化合物的特征离子建立EMIC指纹图谱，并比较不同提取方法。
GC-MS指纹图谱能提供定性信息，确证相应色谱峰的化合物。首次运用EMIC对药材质量进行质量控制推理，可为药材及其制品安全有效的质量控制奠定基础。PR溶剂能简便地从药材中提取挥发油。
该方法具有高效、低成本的优点，GC-MS指纹图谱为药材的归属和质量提供了依据，对推动中药现代化具有重要作用。
