Feng Suo-Min, Gan Zhi-Jie, Zhai Xi-Feng, Fu Peng-Fei, Sun Wen-Ji
Biology and Medicine Key Laboratory of Northwest University, Xi'an 710069, China.
Zhong Yao Cai. 2006 Aug;29(8):792-4.
To evaluate the reborn barks quality, antihypertensive effective component of pinoresnol diglucoside (PDG) in three times reborn barks were determined.
A YWG C18 column (10 microm, 250 x 4.6 mm) was used with a mobile phase of methanol-water (30:75) and flow rate of 1.0 (ml/min). The detective wave-length was set at 277nm and the column temperature at room temperature.
PDG in the first reborn barks are slightly higher than the original ones, and in the second reborn barks are similar with the barks before girdling (the fist reborn barks), but in the third reborn barks, PDG are much lower than the barks before girdling (the second reborn barks).
In order to ensure reborn barks quality, we suggest that the girdling bark regeneration can be made two times, the time between the first and the second girdling is not less than five years. PDG in the third reborn barks should be enhanced.
为评价再生树皮质量，测定了三次再生树皮中松脂醇二葡萄糖苷（PDG）的降压有效成分。
采用YWG C18柱（10μm，250×4.6mm），流动相为甲醇-水（30:75），流速为1.0（ml/min）。检测波长设定为277nm，柱温为室温。
第一次再生树皮中的PDG略高于原树皮，第二次再生树皮中的PDG与环剥前（第一次再生树皮）的树皮相似，但第三次再生树皮中的PDG远低于环剥前（第二次再生树皮）的树皮。
为保证再生树皮质量，建议环剥树皮再生可进行两次，第一次和第二次环剥间隔时间不少于五年。应提高第三次再生树皮中的PDG含量。
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