Biggemann B, Bunse T, Sachsenweger A, Opferkuch W
Lehrstuhl für Medizinische Mikrobiologie und Immunologie der Ruhr-Universität Bochum.
Zentralbl Bakteriol. 1990 Nov;274(2):155-73. doi: 10.1016/s0934-8840(11)80099-x.
Monoclonal antibodies (MAbs) were raised against purified fimbriae isolated from the uropathogenic E. coli strain WF96 (O7:K1:H6:F11rel,F10). This strain expresses at least four different types of fimbriae. 11 MAbs were selected for further characterization. They are directed against epitopes of a fimbrial type which is composed of 19.5 kDa subunits. It resembles type 1 fimbriae with regard to its high resistance to disruption by SDS. The MAbs were tested for crossreactivity to type 1 fimbriae and other fimbriae with known F-serotypes by ELISA. Two of these MAbs, Pili III 2F7 and Pili III 68C5, were directed against an epitope which was also found on MS fimbriae (type 1). Thus type 1 like fimbriae of E. coli WF96 share at least one epitope with MS fimbriae. Nevertheless, the antigenic properties of these two fimbrial types were found not to be completely identical, since all the other 9 MAbs were not crossreactive. The MAbs were not able to inhibit haemagglutination of erythrocytes of different species and thus not directed against adhesive sites of the fimbriae. All the epitopes detected by MAbs were accessible on native fimbriae; some of them were also detectable on denatured fimbrial subunits. Electron micrographs revealed that these epitopes were evenly distributed on the fimbrial organelle.
制备了针对从尿路致病性大肠杆菌菌株WF96（O7:K1:H6:F11rel,F10）中分离出的纯化菌毛的单克隆抗体（MAb）。该菌株表达至少四种不同类型的菌毛。选择了11种单克隆抗体进行进一步表征。它们针对的是由19.5 kDa亚基组成的一种菌毛类型的表位。就其对SDS破坏的高抗性而言，它类似于1型菌毛。通过ELISA测试这些单克隆抗体与1型菌毛和其他具有已知F血清型的菌毛的交叉反应性。其中两种单克隆抗体，菌毛III 2F7和菌毛III 68C5，针对的是在MS菌毛（1型）上也发现的一个表位。因此，大肠杆菌WF96的1型样菌毛与MS菌毛至少共享一个表位。然而，发现这两种菌毛类型的抗原特性并不完全相同，因为所有其他9种单克隆抗体都没有交叉反应性。这些单克隆抗体不能抑制不同物种红细胞的血凝，因此不是针对菌毛的粘附位点。单克隆抗体检测到的所有表位在天然菌毛上都是可及的；其中一些在变性的菌毛亚基上也可检测到。电子显微镜照片显示这些表位均匀分布在菌毛细胞器上。
