de Ree J M, Schwillens P, van den Bosch J F
Infect Immun. 1985 Dec;50(3):900-4. doi: 10.1128/iai.50.3.900-904.1985.
The Escherichia coli P fimbriae F71, F72, F9, and F11 from four cloned strains were purified, and polyclonal antisera were raised in rabbits. Cross-reactions of these antisera with eight different cloned and purified fimbriae were measured in an enzyme-linked immunosorbent assay. These antisera showed a reaction with the homologous fimbriae and also with most heterologous fimbriae. Monoclonal antibodies (MAbs) directed against the same four native fimbriae were produced by the fusion of spleen cells from immunized BALB/c mice with SP2/0 myeloma cells. The resulting four series of MAbs were also screened in an enzyme-linked immunosorbent assay with eight different cloned and purified fimbriae. Four different F71 hybridomas produced MAbs which recognized only epitopes on F71 fimbriae. Two F72 MAbs recognized epitopes on F72 and F9 fimbriae, whereas another F72 MAb recognized an epitope on only F72 fimbriae. Three MAbs raised against F9 reacted only with epitopes on F9 fimbriae. Six MAbs against F11 fimbriae could be divided into two groups: on the one hand two MAbs recognizing F11, pyelonephritis-associated pilus, Pap, and F72 fimbriae and on the other hand four MAbs recognizing F11 and "Clegg" fimbriae. None of the MAbs reacted with 1A or 1C fimbriae. In a hemagglutination inhibition assay it was shown that none of the MAbs produced inhibited the adhesive properties of homologous cloned strains.
从四个克隆菌株中纯化出大肠杆菌P菌毛F71、F72、F9和F11，并在兔体内制备多克隆抗血清。在酶联免疫吸附试验中测定这些抗血清与八种不同克隆和纯化菌毛的交叉反应。这些抗血清与同源菌毛以及大多数异源菌毛都有反应。通过将免疫的BALB/c小鼠的脾细胞与SP2/0骨髓瘤细胞融合，制备了针对相同四种天然菌毛的单克隆抗体（MAb）。所得的四组单克隆抗体也在酶联免疫吸附试验中用八种不同克隆和纯化的菌毛进行筛选。四种不同的F71杂交瘤产生的单克隆抗体仅识别F71菌毛上的表位。两种F72单克隆抗体识别F72和F9菌毛上的表位，而另一种F72单克隆抗体仅识别F72菌毛上的表位。针对F9产生的三种单克隆抗体仅与F9菌毛上的表位反应。针对F11菌毛的六种单克隆抗体可分为两组：一方面，两种单克隆抗体识别F11、肾盂肾炎相关菌毛（Pap）和F72菌毛；另一方面，四种单克隆抗体识别F11和“克莱格”菌毛。没有一种单克隆抗体与1A或1C菌毛反应。在血凝抑制试验中表明，所产生的单克隆抗体均未抑制同源克隆菌株的黏附特性。
