利用针对肠炎沙门氏菌SEF21菌毛的单克隆抗体对沙门氏菌1型菌毛的表位进行表征。

Characterisation of epitopes of type 1 fimbriae of Salmonella using monoclonal antibodies specific for SEF21 fimbriae of Salmonella enteritidis.

作者信息

Sojka M G, Carter M A, Thorns C J

机构信息

Department of Bacteriology, Central Veterinary Laboratory, Surrey, UK.

出版信息

Vet Microbiol. 1998 Jan 16;59(2-3):157-74. doi: 10.1016/s0378-1135(97)00189-2.

DOI:10.1016/s0378-1135(97)00189-2

PMID:9549856

Abstract

Monoclonal antibodies (mAbs) were used to identify and characterise epitopes of type 1 (SEF21) fimbriae of Salmonella enteritidis. The distribution of the epitopes among salmonellas and other enterobacteria was investigated, as well as the influence of growth media and temperatures on their expression. At least four different epitope clusters were identified on SEF21 fimbriae of S. enteritidis. Two of these clusters were associated with fimbrial haemagglutinins that were either common to all salmonellae tested, or restricted only to S. enteritidis and S. dublin. The four epitope clusters were identified on type 1 fimbriae of most Salmonella serotypes, as well as non-haemagglutinating type 2 fimbriae of S. pullorum and S. gallinarum, and on many other enterobacterial species. The expression of the epitopes was affected by growth conditions.

摘要

单克隆抗体（mAbs）用于鉴定和表征肠炎沙门氏菌1型（SEF21）菌毛的表位。研究了这些表位在沙门氏菌和其他肠道细菌中的分布，以及生长培养基和温度对其表达的影响。在肠炎沙门氏菌的SEF21菌毛上鉴定出至少四个不同的表位簇。其中两个簇与菌毛血凝素相关，这些血凝素要么在所有测试的沙门氏菌中都有，要么仅局限于肠炎沙门氏菌和都柏林沙门氏菌。在大多数沙门氏菌血清型的1型菌毛上、鸡白痢沙门氏菌和鸡伤寒沙门氏菌的非血凝性2型菌毛上以及许多其他肠道细菌物种上都鉴定出了这四个表位簇。表位的表达受生长条件的影响。