Birkholz S, Goroncy-Bermes P, Schönermark M, Hänsch G M, Opferkuch W
Abt. Med. Mikrobiologie und Immunologie, Ruhr-Universität Bochum.
Zentralbl Bakteriol. 1990 Nov;274(2):183-94. doi: 10.1016/s0934-8840(11)80101-5.
The cross reactivity of the murine monoclonal antibody PM II 40 against human glomeruli with streptococcal type 1 M protein was investigated. The antibody PM II 40 recognized a protective epitope on a glomerulonephritis-associated M type 1 strain. A 23 kD streptococcal surface protein extracted by limited pepsin digestion reacted with PM II 40 in the Western immunoblot. The aminoterminal sequence of this peptide was identical to the known aminoterminal amino acid sequence of type 1 M protein. Human renal podocytes carry the cross-reactive antigen of the antibody PM II 40 as could be shown by electron microscopy. The podocytes cultured from isolated human glomeruli showed a fibrillar pattern with the antibody in the immunofluorescence test. An anti-vimentin antibody and the antibody PM II 40 recognized the same proteins with molecular weights of 54, 52 and 43 kD of SDS-extracted isolated human glomeruli suggesting that vimentin is the glomerular antigen. The antibody PM II 40 not only react with vimentin but also with desmin suggesting that the recognized epitopes are distinct from that described by Kraus et al. for vimentin and type 1 M protein.
研究了鼠单克隆抗体PM II 40与人肾小球及1型链球菌M蛋白的交叉反应性。抗体PM II 40识别与肾小球肾炎相关的1型菌株上的一个保护性表位。经有限胃蛋白酶消化提取的一种23 kD链球菌表面蛋白在Western免疫印迹中与PM II 40发生反应。该肽的氨基末端序列与已知的1型M蛋白氨基末端氨基酸序列相同。电子显微镜显示，人肾足细胞携带抗体PM II 40的交叉反应抗原。在免疫荧光试验中，从分离的人肾小球培养的足细胞与该抗体呈现出纤维状模式。一种抗波形蛋白抗体和抗体PM II 40识别经SDS提取的分离人肾小球中分子量为54、52和43 kD的相同蛋白质，提示波形蛋白是肾小球抗原。抗体PM II 40不仅与波形蛋白反应，还与结蛋白反应，提示所识别的表位与Kraus等人描述的波形蛋白和1型M蛋白的表位不同。
