Goroncy-Bermes P, Dale J B, Beachey E H, Opferkuch W
Department of Medical Microbiology and Immunology, Ruhr-Universität Bochum, Federal Republic of Germany.
Infect Immun. 1987 Oct;55(10):2416-9. doi: 10.1128/iai.55.10.2416-2419.1987.
Two murine monoclonal antibodies (immunoglobulin M) evoked against human kidney tissue were examined for cross-reactivity with group A streptococci. A glomerulus-specific antibody, PMII.40.H.2, cross-reacted in an enzyme-linked immunosorbent assay with purified pepsin-extracted M proteins from type 6 and 12 streptococci, but not type 1, 3, 5, 19, and 24 M proteins. The cross-reactive monoclonal antibody also opsonized type 6 and 12 streptococci, indicating that it was directed against protective M protein epitopes that were exposed on the surfaces of these organisms. A control antibody, which was tubule specific, did not cross-react with any of the purified M proteins, nor did it opsonize any of the serotypes of streptococci tested. Western immunoblot experiments identified the glomerular cross-reactive antigen as a 43-kilodalton protein. The results demonstrate that M protein of group A streptococci and glomerular antigens of the human kidney possess cross-reactive determinants.
检测了两种针对人肾组织产生的鼠单克隆抗体（免疫球蛋白M）与A组链球菌的交叉反应性。一种肾小球特异性抗体PMII.40.H.2在酶联免疫吸附试验中与从6型和12型链球菌中纯化的经胃蛋白酶提取的M蛋白发生交叉反应，但与1型、3型、5型、19型和24型M蛋白不发生交叉反应。这种交叉反应性单克隆抗体也能调理6型和12型链球菌，表明它针对的是这些细菌表面暴露的保护性M蛋白表位。一种肾小管特异性对照抗体与任何纯化的M蛋白均无交叉反应，也不能调理所检测的任何链球菌血清型。蛋白质免疫印迹实验确定肾小球交叉反应性抗原为一种43千道尔顿的蛋白质。结果表明，A组链球菌的M蛋白与人肾的肾小球抗原有交叉反应性决定簇。
