Wang Hong-Kun, Zhu Ping, Li Xiao-Yan, Zheng Zhao-Hui, Jia Jun-Feng, Ding Jin, Fan Chun-Mei
Department of Clinical Immunology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2006 Nov;22(6):742-4.
To investigate the modulation of rhC II 250-270 peptide on special humoral immune response in the course of oral administration.
ELISA was used to determine antigen-specific antibodies in mice sera. The frequency of anti-C II and anti-C II (250-270) antibody-forming spleen cells was measured by ELISPOT.
The level of C II- and C II (250-270)- specific IgG in serum from the mice fed with rhC II (250-270) were (0.82+/-0.02) and (0.84+/-0.04) respectively, and lower significantly than those of collagen-induced arthritis (CIA) control group. The anti-C II (250-270) antibody responses were suppressed obviously (P<0.01). The frequency of antibody-forming cells in the spleen from rhC II (250-270)-fed mice were (158+/-9 counts/well) and (181+/-10 counts/well) respectively, and also were reduced significantly when compared with that in CIA control group (247+/-16 counts/well)(P<0.05).
Oral rhC II (250-270) could induce specific suppression of humoral responds in CIA. These findings together with a better understanding of the mechanisms of oral tolerance and regulation of humoral immune response in CIA, will help the development of innovative therapeutic intervention for rheumatoid arthritis.
研究重组人CⅡ250 - 270肽在口服给药过程中对特异性体液免疫反应的调节作用。
采用ELISA法测定小鼠血清中的抗原特异性抗体。通过ELISPOT检测抗CⅡ和抗CⅡ（250 - 270）抗体形成脾细胞的频率。
喂食重组人CⅡ（250 - 270）的小鼠血清中CⅡ和CⅡ（250 - 270）特异性IgG水平分别为（0.82±0.02）和（0.84±0.04），显著低于胶原诱导性关节炎（CIA）对照组。抗CⅡ（250 - 270）抗体反应明显受到抑制（P<0.01）。喂食重组人CⅡ（250 - 270）的小鼠脾脏中抗体形成细胞的频率分别为（158±9个/孔）和（181±10个/孔），与CIA对照组（247±16个/孔）相比也显著降低（P<0.05）。
口服重组人CⅡ（250 - 270）可诱导对CIA中体液反应的特异性抑制。这些发现以及对口服耐受机制和CIA中体液免疫反应调节的更好理解，将有助于类风湿关节炎创新治疗干预措施的开发。
