Zhang Xingyuan, Zhang Lirong, Li Yuanwei, Hua Youlu, Li Yangde, Li Weirong, Li Wei
School of Mechanical Engineering, Liaoning Technical University, Fuxin 123000, China.
Institute of Advanced Wear & Corrosion Resistant and Functional Materials, Jinan University, Guangzhou 510632, China.
Materials (Basel). 2019 Apr 18;12(8):1274. doi: 10.3390/ma12081274.
Hierarchically porous hydroxyapatite (HHA) scaffolds were synthesized by template-assisted sol-gel chemistry. Polyurethane foam and a block copolymer were used as templates for inducing hierarchically porous structures. The HHA scaffolds exhibited open porous structures with large pores of 400-600 µm and nanoscale pores of ~75 nm. In comparison with conventional hydroxyapatite (CHA), HHA scaffolds exhibited significantly higher surface areas and increased protein adsorption for bovine serum albumin and vitronectin. Both the HHA and CHA scaffolds exhibited well in vitro biocompatibility. After 1 day, Saos-2 osteoblast-like cells bound equally well to both HHA and CHA scaffolds, but after 7 days in culture, cell proliferation was significantly greater on the HHA scaffolds ( < 0.01). High surface area and hierarchical porous structure contributed to the selective enhancement of osteoblast proliferation on the HHA scaffolds.
通过模板辅助溶胶-凝胶化学合成了具有分级多孔结构的羟基磷灰石（HHA）支架。聚氨酯泡沫和嵌段共聚物被用作诱导分级多孔结构的模板。HHA支架呈现出开放的多孔结构，具有400-600μm的大孔和约75nm的纳米级孔。与传统羟基磷灰石（CHA）相比，HHA支架表现出显著更高的表面积，并且对牛血清白蛋白和玻连蛋白的蛋白质吸附增加。HHA和CHA支架在体外均表现出良好的生物相容性。培养1天后，Saos-2成骨样细胞与HHA和CHA支架的结合效果相同，但培养7天后，HHA支架上的细胞增殖明显更大（<0.01）。高表面积和分级多孔结构有助于选择性增强HHA支架上的成骨细胞增殖。
