Likeufack A C Lékané, Brun R, Fomena A, Truc P
Organisation de Coordination pour la Lutte Contre les Endémies en Afrique Centrale, Yaoundé, Cameroon.
Acta Trop. 2006 Nov;100(1-2):11-6. doi: 10.1016/j.actatropica.2006.09.003. Epub 2006 Oct 31.
The situation of human African trypanosomiasis remains serious with one of the main threats being the increasing number of relapses or treatment failures after melarsoprol treatment. In order to investigate and to compare drug sensitivities of trypanosomes isolated at different time periods and in different locations, two sets of Trypanosoma brucei gambiense strains were used. One set was isolated in the time period 1960-1981 and the other one in 1995-2004 from different locations of West and Central Africa. These isolates were not selected based on the treatment outcome but on availability. The drug sensitivity profile for all available drugs in use and the diamidine compound DB75 was established. IC(50) values were not significantly different between the "old" and "new" stocks. No indications for emerging drug resistance to any drug could be observed. The results indicate a relative stability of in vitro sensitivity of T. b. gambiense to trypanocidal drugs in space (West and Central Africa) and time (1960-2004).
人类非洲锥虫病的形势依然严峻，主要威胁之一是美拉胂醇治疗后复发或治疗失败的病例不断增加。为了调查和比较在不同时期和不同地点分离出的锥虫的药物敏感性，使用了两组布氏冈比亚锥虫菌株。一组于1960年至1981年期间分离，另一组于1995年至2004年期间从西非和中非的不同地点分离。这些分离株不是根据治疗结果而是根据可获得性选择的。确定了所有现有使用药物和二脒化合物DB75的药物敏感性概况。“旧”菌株和“新”菌株的半数抑制浓度（IC50）值没有显著差异。未观察到对任何药物出现耐药性的迹象。结果表明，布氏冈比亚锥虫在体外对杀锥虫药物的敏感性在空间（西非和中非）和时间（1960年至2004年）上具有相对稳定性。
