通过代谢工程改造的枯草芽孢杆菌菌株生产木糖醇。
Production of xylitol by metabolically engineered strains of Bacillus subtilis.
作者信息
Povelainen Mira, Miasnikov Andrei N
机构信息
Danisco Innovation, Sokeritehtaantie 20, 02460 Kantvik, Finland.
出版信息
J Biotechnol. 2007 Jan 30;128(1):24-31. doi: 10.1016/j.jbiotec.2006.09.008. Epub 2006 Sep 23.
Xylitol-phosphate dehydrogenase (XPDH) genes from several Gram-positive bacteria were isolated and expressed in Bacillus subtilis. The substrate specificities of the recombinant XPDH enzymes were compared and it was found that the XPDH enzymes of Lactobacillus rhamnosus and Clostridium difficile had the highest selectivity towards D-xylulose 5-phosphate. Expression of these two XPDH enzymes in D-ribulose and D-xylulose producing B. subtilis strain resulted in strains of B. subtilis capable of converting D-glucose into xylitol at around 23% yield.
从几种革兰氏阳性细菌中分离出木糖醇磷酸脱氢酶（XPDH）基因，并在枯草芽孢杆菌中表达。比较了重组XPDH酶的底物特异性，发现鼠李糖乳杆菌和艰难梭菌的XPDH酶对5-磷酸-D-木酮糖具有最高的选择性。在生产D-核糖和D-木酮糖的枯草芽孢杆菌菌株中表达这两种XPDH酶，得到了能够将D-葡萄糖转化为木糖醇的枯草芽孢杆菌菌株，转化率约为23%。
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